Rabbit anti-Human, Mouse NECAB3 Polyclonal Antibody | anti-NECAB3 antibody

NECAB3 Antibody

Cat. Num. AAA9506020

• Gene Names: NECAB3; NIP1; XB51; STIP3; EFCBP3; SYTIP2; APBA2BP; dJ63M2.4; dJ63M2.5
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: NECAB3; Polyclonal Antibody; NECAB3 Antibody; anti-NECAB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: NECAB3 Antibody detects endogenous levels of total NECAB3 protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 203

• Applicable Applications for anti-NECAB3 antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:10000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human NECAB3.
• Immunogen Range: 321-370
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells, using NECAB3 Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from HepG2, COLO, and HT-29 cells, using NECAB3 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 149158725
• NCBI GeneID: 63941
• NCBI Accession #: NP_112508.3
• NCBI GenBank Nucleotide #: NM_031231.3
• UniProt Accession #: Q96P71; Q5JWF5; Q5JWF6; Q5JWF7; Q86VV1; Q9H433; Q9H8G8; Q9HBW7; Q9HCQ9; A8K780; E1P5N2
• Molecular Weight: 44 kDa
• NCBI Official Full Name: N-terminal EF-hand calcium-binding protein 3 isoform 1
• NCBI Official Synonym Full Names: N-terminal EF-hand calcium binding protein 3
• NCBI Official Symbol: NECAB3
• NCBI Official Synonym Symbols: NIP1; XB51; STIP3; EFCBP3; SYTIP2; APBA2BP; dJ63M2.4; dJ63M2.5
• NCBI Protein Information: N-terminal EF-hand calcium-binding protein 3
• UniProt Protein Name: N-terminal EF-hand calcium-binding protein 3
• Protein Family: N-terminal EF-hand calcium-binding protein
• UniProt Gene Name: NECAB3
• UniProt Synonym Gene Names: APBA2BP; NIP1; SYTIP2; XB51

NCBI Description

The protein encoded by this gene interacts with the amino-terminal domain of the neuron-specific X11-like protein (X11L), inhibits the association of X11L with amyloid precursor protein through a non-competitive mechanism, and abolishes the suppression of beta-amyloid production by X11L. This protein, together with X11L, may play an important role in the regulatory system of amyloid precursor protein metabolism and beta-amyloid generation. The protein is phosphorylated by NIMA-related expressed kinase 2, and localizes to the Golgi apparatus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibits the interaction of APBA2 with amyloid-beta precursor protein (APP), and hence allows formation of amyloid-beta. May enhance the activity of HIF1A and thus promote glycolysis under normoxic conditions; the function requires its ABM domain and may implicate the stabilization of the interaction between HIF1AN and APBA3.