Transportin 1 (TNPO1) Recombinant Protein | TNPO1 recombinant protein

Recombinant Transportin 1 (TNPO1)

Cat. Num. AAA2011753

• Gene Names: TNPO1; TRN; KPNB2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Transportin 1 (TNPO1); Recombinant Transportin 1 (TNPO1); TNPO1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-PLLECLSS VATALQSGFL PYCEPVYQRC VNLVQKTLAQ AMLNNAQPDQ YEAPDKDFMI VALDLLSGLA EGLGGNIEQL VARSNILTLM YQCMQDKMPE VRQSSFALLG DLTKACFQHV KPCIADFMPI LGTNLNPEFI SVCNNATWAI GEISIQMGIE MQPYIPMVLH QLVEIINRPN TPKTLLENTA ITIGRLGYVC PQEVAPMLQQ FIRPWCTSLR NIRDNEEKDS AFRGICTMIS VNPSGV
• Sequence Length: 890

• Applicable Applications for TNPO1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Bos taurus; Bovine (Cattle)
• Expression System: Prokaryotic expression
• Residues: Pro593~Val836 (Accession # Q3SYU7) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 115496061
• NCBI GeneID: 767620
• NCBI Accession #: NP_001070008.1
• NCBI GenBank Nucleotide #: NM_001076540.1
• UniProt Accession #: Q3SYU7
• Molecular Weight: 28.4kDa
• NCBI Official Full Name: transportin-1
• NCBI Official Symbol: TNPO1
• NCBI Official Synonym Symbols: TRN; KPNB2
• NCBI Protein Information: transportin-1; importin beta-2; karyopherin beta-2; karyopherin (importin) beta 2
• UniProt Protein Name: Transportin-1
• UniProt Gene Name: TNPO1
• UniProt Synonym Gene Names: KPNB2
• UniProt Entry Name: TNPO1_BOVIN

Uniprot Description

Function: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev

By similarity. Mediates nuclear import of ADAR/ADAR1 in a RanGTP-dependent manner

By similarity.

Subunit structure: Binds HNRPA1, HNRPA2, HNRNPDL, RPL23A, RPS7, RPL5, RAN and SRP19. Interacts with H2A, H2B, H3 and H4 histones

By similarity. Interacts with ADAR/ADAR1 (via DRBM 3 domain). Interacts with SNAI1 (via zinc fingers); the interaction mediates SNAI1 nuclear import. Interacts with SNAI2 (via zinc fingers)

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus

By similarity.

Sequence similarities: Belongs to the importin beta family.Contains 8 HEAT repeats.Contains 1 importin N-terminal domain.

Sequence caution: The sequence AAI03375.1 differs from that shown. Reason: Erroneous initiation.