alpha amylase/AMY2A Recombinant Protein | AMY2A recombinant protein

alpha amylase/AMY2A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121365

• Gene Names: AMY2A; PA; AMY2; AMY2B
• Purity: >85% as determined by SDS-PAGE
• Synonyms: alpha amylase/AMY2A; alpha amylase/AMY2A Protein; Human; Recombinant (His Tag); Human AMY2A/Alpha-amylase Protein(His Tag); AMY2 Protein; AMY2A Protein; PA Protein; amylase; alpha 2A (pancreatic); AMY2A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu511

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 16
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human AMY2A (P04746)(Met1-Leu511) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for AMY2A recombinant protein

Background: Alpha-amylase is the major form of amylase found in humans and other mammals. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. Alpha-amylase hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Amylases is widely expressed and is most prominent in pancreatic juice and saliva, each of which has its own isoform of human alpha-amylase. They behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies.

References

Abe A, et al. (2005) Complexes of Thermoactinomyces vulgaris R-47 Alpha-amylase/AMY2A 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with alpha-(1,6) glycosidic linkages. FEBS J. 272(23):6145-53.Aghajari, N, et al. (1998) Crystal structures of the psychrophilic Alpha-amylase/AMY2A from Alteromonas haloplanctis in its native form and complexed with an inhibitor. Protein Sci. 7(3): 564-72.Ramasubbu, N, et al. (1996) Structure of Human Salivary -Amylase at 1.6 Resolution: Implications for its Role in the Oral Cavity. Acta Crystallographica Section D Biological Crystallography. 52(3):435-46.

NCBI and Uniprot Product Information

• NCBI GI #: 529397
• NCBI GeneID: 279
• UniProt Accession #: P04746; Q9UBH3
• Molecular Weight: 57,707 Da
• NCBI Official Full Name: amylase
• NCBI Official Synonym Full Names: amylase, alpha 2A (pancreatic)
• NCBI Official Symbol: AMY2A
• NCBI Official Synonym Symbols: PA; AMY2; AMY2B
• NCBI Protein Information: pancreatic alpha-amylase; glycogenase; found in the pancreas; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase, pancreatic, alpha-2A; 1,4-alpha-D-glucan glucanohydrolase
• UniProt Protein Name: Pancreatic alpha-amylase
• UniProt Gene Name: AMY2A
• UniProt Synonym Gene Names: PA
• UniProt Entry Name: AMYP_HUMAN

NCBI Description

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Uniprot Description

AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Protein type: EC 3.2.1.1; Secreted; Hydrolase; Secreted, signal peptide; Carbohydrate Metabolism - starch and sucrose

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular space; extracellular region

Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding

Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis