CAMKV recombinant protein

CAMKV Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8121131

• Gene Names: CAMKV; 1G5; VACAMKL
• Purity: >80% as determined by SDS-PAGE
• Synonyms: CAMKV; CAMKV Protein; Human; Recombinant (His & GST Tag); Human CAMKV Protein (His & GST Tag); 1G5 Protein; VACAMKL Protein; CaM kinase-like vesicle-associated; CAMKV recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser501

• Species: Human
• Activity: No Kinase Activity
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human CAMKV (NP_076951.2)(Met 1-Ser 501) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CAMKV recombinant protein

Background: CaM kinase-like vesicle-associated protein, also known as CAMKV, is a peripheral membrane protein and Cytoplasmic vesicle membrane protein which belongs to theprotein kinase superfamily and CAMK Ser/Thr protein kinase family. CAMKV contains oneprotein kinase domain. It is predominantly observed in association with the plasma membrane of soma and in neurites, both axons and dendrites. CAMKV may be associated with vesicular structures. It does not appear to have detectable kinase activity. Protein kinases are a group of enzymes that move a phosphate group onto proteins, in a process called phosphorylation. Protein kinases function as an on/off switch for many cellular processes, including metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. They also function in embryonic development, physiological responses, and in the nervous and immune system. Abnormal phosphorylation causes many human diseases, including cancer, and drugs that affect phosphorylation can treat those diseases. The protein kinase domain is a structurally conserved protein domain containing the catalytic function of protein kinases. Protein kinases play a role in a mulititude of cellular processes, including division, proliferation, apoptosis, and differentiation. Phosphorylation usually results in a functional change of the target protein by changing enzyme activity, cellular location, or association with other proteins.

Product Categories/Family for CAMKV recombinant protein

EGFR Signaling Pathway

References

Hunter T, et al.,1988, Science. 241 (4861): 42-51. Wiemann S., et al., 2001, Genome Res. 11:422-435. G. Manning, et al., 2002, Science 6. 298:1912-1934. Manning G, et al.,2002, Science. 298 (5600): 1912-34. Ota T., et al., 2004, Nat. Genet. 36:40-45.

NCBI and Uniprot Product Information

• NCBI GI #: 83776596
• NCBI GeneID: 79012
• NCBI Accession #: NP_076951.2
• NCBI GenBank Nucleotide #: NM_024046.3
• UniProt Accession #: Q8NCB2; Q6FIB8; Q8NBS8; Q8NC85; Q8NDU4; Q8WTT8; Q9BQC9; Q9H0Q5; A6NFD4
• Molecular Weight: 54,354 Da
• NCBI Official Full Name: caM kinase-like vesicle-associated protein
• NCBI Official Synonym Full Names: CaM kinase-like vesicle-associated
• NCBI Official Symbol: CAMKV
• NCBI Official Synonym Symbols: 1G5; VACAMKL
• NCBI Protein Information: caM kinase-like vesicle-associated protein; vesicle-associated calmodulin-binding protein
• UniProt Protein Name: CaM kinase-like vesicle-associated protein
• Protein Family: CaM kinase-like vesicle-associated protein
• UniProt Gene Name: CAMKV
• UniProt Entry Name: CAMKV_HUMAN

Uniprot Description

VACAMKL: Does not appear to have detectable kinase activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; CAMK group; CAMK-Unique family

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cytoplasmic vesicle membrane; plasma membrane

Molecular Function: calmodulin binding; ATP binding; protein kinase activity

Biological Process: protein amino acid phosphorylation