HDAC4 recombinant protein

HDAC4 Protein, Human, Recombinant (aa 612-1084)

Cat. Num. AAA8120976

• Gene Names: HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
• Purity: >90% as determined by SDS-PAGE
• Synonyms: HDAC4; HDAC4 Protein; Human; Recombinant (aa 612-1084); Human HDAC4 Protein (aa 612-1084); AHO3 Protein; BDMR Protein; HA6116 Protein; HD4 Protein; HDAC-4 Protein; HDAC-A Protein; HDACA Protein; histone deacetylase 4; HDAC4 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris,500mM NaCl, pH7.4, 10%gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met612-Leu1084

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the human HDAC4 (NP_006028.2)(Met612-Leu1084) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for HDAC4 recombinant protein

Background: HDAC4 (histone deacetylase 4), belongs to class II of the histone deacetylase/AcuC/APhA family. Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general, they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2, and 4, which are closely related to Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is affected by protein-protein interactions and by the class to which they belong. HDACs have a role in cell growth arrest, differentiation, and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents. HDAC4 possesses histone deacetylase activity and represses transcription when tethered to a promoter. It does not bind DNA directly but through transcription factors MEF2C and MEF2D. HDAC4 seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Product Categories/Family for HDAC4 recombinant protein

Cancer Drug Targets; Notch Signaling

References

Geng H, et al. (2011) HDAC4 protein regulates HIF1alpha protein lysine acetylation and cancer cell response to hypoxia. J Biol Chem. 286(44):38095-102.Yuan JH, et al. (2011) The histone deacetylase 4/SP1/microrna-200a regulatory network contributes to aberrant histone acetylation in hepatocellular carcinoma. Hepatology. 54(6):2025-35.Cernotta N, et al. (2011) Ubiquitin-dependent degradation of HDAC4, a new regulator of random cell motility. Mol Biol Cell. 22(2):278-89.

NCBI and Uniprot Product Information

• NCBI GI #: 153085395
• NCBI GeneID: 9759
• NCBI Accession #: NP_006028.2
• NCBI GenBank Nucleotide #: NM_006037.3
• UniProt Accession #: P56524; Q9UND6
• Molecular Weight: 119,040 Da
• NCBI Official Full Name: histone deacetylase 4
• NCBI Official Synonym Full Names: histone deacetylase 4
• NCBI Official Symbol: HDAC4
• NCBI Official Synonym Symbols: HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
• NCBI Protein Information: histone deacetylase 4; histone deacetylase A
• UniProt Protein Name: Histone deacetylase 4
• UniProt Gene Name: HDAC4
• UniProt Synonym Gene Names: KIAA0288; HD4
• UniProt Entry Name: HDAC4_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC4: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Protein type: Nuclear receptor co-regulator; Hydrolase; Deacetylase; EC 3.5.1.98

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: nucleoplasm; transcriptional repressor complex; cytoplasm; histone deacetylase complex; actomyosin; neuromuscular junction; Z disc; cytosol; nucleus; A band

Molecular Function: potassium ion binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; transcription activator binding; NAD-dependent histone deacetylase activity (H3-K14 specific); zinc ion binding; sequence-specific DNA binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; protein kinase binding; transcription factor binding; transcription corepressor activity

Biological Process: cardiac muscle hypertrophy; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; response to denervation involved in regulation of muscle adaptation; regulation of gene expression, epigenetic; negative regulation of cell proliferation; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; negative regulation of glycolysis; inflammatory response; skeletal development; response to drug; nervous system development; regulation of skeletal muscle fiber development; B cell activation; transcription, DNA-dependent; negative regulation of transcription factor activity; histone deacetylation; osteoblast development; chromatin remodeling; positive regulation of protein sumoylation; regulation of protein binding; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent