MIP-5 Recombinant Protein | CCL15 recombinant protein

MIP-5 Protein, Human, Recombinant (aa 46-113, His Tag)

Cat. Num. AAA8120560

• Gene Names: CCL15; LKN1; NCC3; SY15; HCC-2; LKN-1; MIP-5; NCC-3; SCYL3; MIP-1D; MRP-2B; SCYA15; HMRP-2B; MIP-1 delta
• Purity: >90% as determined by SDS-PAGE
• Synonyms: MIP-5; MIP-5 Protein; Human; Recombinant (aa 46-113; His Tag); Human CCL15/MIP-5/MIP-1 delta Protein (aa 46-113; HCC-2 Protein; HMRP-2B Protein; LKN-1 Protein; LKN1 Protein; MIP-1 delta Protein; MIP-1D Protein; MRP-2B Protein; NCC-3 Protein; NCC3 Protein; SCYA15 Protein; SCYL3 Protein; SY15 Protein; chemokine (C-C motif) ligand 15; CCL15 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser46-Ile113

• Species: Human
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the amino acids (Ser 46 -Ile 113) of human CCL15 (Q16663-1) was expressed, with a polyhistide tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for CCL15 recombinant protein

Background: Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 15 (CCL15), also known as leukotactin-1, MIP5, MIP1 and HCC-2, is a small cytokine belonging to the C-C chemokine family. CCL15 is prevantly expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3.

References

Laing KJ, et al. (2004) Chemokines. Developmental and comparative immunology. 28(5): 443-60.Youn, et al. (1997) Molecular cloning of leukotactin-1: a novel human beta-chemokine, a chemoattractant for neutrophils, monocytes, and lymphocytes, and a potent agonist at CC chemokine receptors 1 and 3. J Immun. 159: 5201-5.Coulin, et al. (1997) Characterization of macrophage inflammatory protein-5/human CC cytokine-2, a member of the macrophage-inflammatory-protein family of chemokines. Europ J Biochem. 248: 507-15.

NCBI and Uniprot Product Information

• NCBI GI #: 597709818
• NCBI GeneID: 6359
• NCBI Accession #: NP_116741.2
• NCBI GenBank Nucleotide #: NM_032965.5
• UniProt Accession #: Q16663; Q9UM74; B2RU34; E1P651
• Molecular Weight: 12,248 Da
• NCBI Official Full Name: C-C motif chemokine 15 preproprotein
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 15
• NCBI Official Symbol: CCL15
• NCBI Official Synonym Symbols: LKN1; NCC3; SY15; HCC-2; LKN-1; MIP-5; NCC-3; SCYL3; MIP-1D; MRP-2B; SCYA15; HMRP-2B; MIP-1 delta
• NCBI Protein Information: C-C motif chemokine 15; chemokine CC-2; leukotactin 1; macrophage inflammatory protein 5; new CC chemokine 3; small inducible cytokine subfamily A (Cys-Cys), member 15; small-inducible cytokine A15
• UniProt Protein Name: C-C motif chemokine 15
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL15
• UniProt Synonym Gene Names: MIP5; NCC3; SCYA15; HCC-2; LKN-1; MIP-5
• UniProt Entry Name: CCL15_HUMAN

NCBI Description

This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14). [provided by RefSeq, Jan 2013]

Uniprot Description

CCL15: Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: heparin binding; chemokine activity; receptor binding; chemoattractant activity

Biological Process: cellular calcium ion homeostasis; cell-cell signaling; positive chemotaxis; immune response; signal transduction; chemotaxis