MAP1D Active Protein | METAP1D active protein

MAP1D Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120550

• Gene Names: METAP1D; MAP1D; MAP 1D; Metap1l; MetAP 1D
• Purity: >92% as determined by SDS-PAGE
• Synonyms: MAP1D; MAP1D Protein; Human; Recombinant (His Tag); Human MAP1D/Methionine Aminopeptidase 1D Protein (His Tag); MAP 1D Protein; MetAP 1D Protein; Metap1l Protein; methionyl aminopeptidase type 1D (mitochondrial); METAP1D active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Arg44-Ala335

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human MAP1D (NP_954697.1)(Arg 44-Ala 335) was expressed, with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC The resulting GP-AMC is cleaved by human DPPIV/CD26 The specific activity is >30pmoles/min/ug.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for METAP1D active protein

Background: Methionine aminopeptidase 1D, also known as MAP1D, is a member of the peptidase M24A family. N-terminal methionine removal is an important cellular process required for proper biological activity, subcellular localization, and eventual degradation of many proteins. The enzymes that catalyze this reaction are called Methionine aminopeptidases (MAPs). MAP1D is overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). Downregulation of MAP1D expression by shRNA in HCT-116 colon carcinoma cells reduces anchorage-independent growth in soft agar. MAP1D binds two cobalt ions per subunit. The true nature of the physiological cofactor is under debate. MAP1D is also active with zinc, manganese, or divalent ions. MAP1D removes the amino-terminal methionine from nascent proteins. It may also play an important role in colon tumorigenesis.

References

Serero A., et al.,(2003), An unusual peptide deformylase features in the human mitochondrial N-terminal methionine excision pathway. J. Biol. Chem. 278:52953-52963.Leszczyniecka M., et al., (2006), MAP1D, a novel methionine aminopeptidase family member is overexpressed in colon cancer.Oncogene 25:3471-3478.Vaca Jacome A.S., et al.,(2015), N-terminome analysis of the human mitochondrial proteome.Proteomics 15:2519-2524.

NCBI and Uniprot Product Information

• NCBI GI #: 40385867
• NCBI GeneID: 254042
• NCBI Accession #: NP_954697.1
• NCBI GenBank Nucleotide #: NM_199227.1
• UniProt Accession #: Q6UB28; Q1WNX3
• Molecular Weight: 37,088 Da
• NCBI Official Full Name: methionine aminopeptidase 1D, mitochondrial
• NCBI Official Synonym Full Names: methionyl aminopeptidase type 1D (mitochondrial)
• NCBI Official Symbol: METAP1D
• NCBI Official Synonym Symbols: MAP1D; MAP 1D; Metap1l; MetAP 1D
• NCBI Protein Information: methionine aminopeptidase 1D, mitochondrial; CDS of metAP-3 within PCR fragment; MAP 1D; metAP 1D; methionyl aminopeptidase type 1D, mitochondrial; peptidase M 1D
• UniProt Protein Name: Methionine aminopeptidase 1D, mitochondrial
• UniProt Gene Name: METAP1D
• UniProt Entry Name: MAP12_HUMAN

NCBI Description

The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM, Mar 2008]

Uniprot Description

MAP1D: Removes the N-terminal methionine from nascent proteins. May play a role in colon tumorigenesis. Belongs to the peptidase M24A family.

Protein type: EC 3.4.11.18; Protease; Mitochondrial

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: mitochondrion

Molecular Function: metalloexopeptidase activity; metal ion binding; aminopeptidase activity

Biological Process: peptidyl-methionine modification; proteolysis; N-terminal protein amino acid modification