Testing Data
(Flow cytometric analysis of Human Alpha-fetoprotein expression on HepG2 cells. The cells were treated according to manufacturer's manual, stained with PE-conjugated anti-Human Alpha-fetoprotein. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Mouse anti-Human Alpha-fetoprotein Monoclonal Antibody | anti-AFP antibody
Anti-Alpha-fetoprotein Antibody (PE), Mouse Monoclonal
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
Alpha-fetoprotein; Monoclonal Antibody; Anti-Alpha-fetoprotein Antibody (PE); Mouse Monoclonal; AFP/alpha-fetoprotein Antibody (PE); Mouse MAb; Anti-AFPD Antibody; Anti-Alpha-fetoprotein Antibody; Anti-FETA Antibody; Anti-HPAFP Antibody; alpha-fetoprotein; anti-AFP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
35
Specificity
Human AFP/alpha-fetoprotein
Purity/Purification
Protein A
Form/Format
Liquid. Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
5ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-AFP antibody
Flow Cytometry (FC/FACS)
Immunogen
Recombinant Human AFP/alpha-fetoprotein protein
Conjugation
PE
Preparation
This product is a recombinant monoclonal antibody expressed from HEK293 cells. Then conjugated with PE under optimum conditions, the unreacted PE was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze!
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Testing Data
(Flow cytometric analysis of Human Alpha-fetoprotein expression on HepG2 cells. The cells were treated according to manufacturer's manual, stained with PE-conjugated anti-Human Alpha-fetoprotein. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Testing Data
(Flow cytometric analysis of Human Alpha-fetoprotein expression on HepG2 cells. The cells were treated according to manufacturer's manual, stained with PE-conjugated anti-Human Alpha-fetoprotein. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-AFP antibody
Background: Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).
References
Mizejewski GJ. (2001) Alpha-fetoprotein Structure and Function: Relevance to Isoforms, Epitopes, and Conformational Variants. Exp Biol Med. 226(5): 377-408.
Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid: comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid: comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
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Product Notes
The AFP (Catalog #AAA8119041) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Alpha-fetoprotein Antibody (PE), Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-fetoprotein can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the AFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-fetoprotein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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