Testing Data
IL10RB elisa pair set
IL10RB Matched ELISA Antibody Pair Set, Mouse
Synonyms
IL10RB; IL10RB Matched ELISA Antibody Pair Set; Mouse; Mouse IL10RB/IL-10RB ELISA Pair Set; interleukin 10 receptor; beta; 6620401D04Rik Matched ELISA Antibody Pair Set; AI528744 Matched ELISA Antibody Pair Set; CRF2-4 Matched ELISA Antibody Pair Set; Crfb4 Matched ELISA Antibody Pair Set; D16H21S58 Matched ELISA Antibody Pair Set; D21S58h Matched ELISA Antibody Pair Set; IL-10R2 Matched ELISA Antibody Pair Set; Il10r2 Matched ELISA Antibody Pair Set; IL10RB elisa pair set
Specificity
Quantitative determination of Mouse IL10RB
Assay Type
Solid Phase Sandwich ELISA
Detection Range
39.06-2500pg/mL
Sensitivity
39.06pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of rabbit anti-Mouse IL10RB/IL-10RB monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse IL10RB/IL-10RB monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 45 ng of recombinant Mouse IL10RB/IL-10RB. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse IL10RB/IL-10RB monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 45 ng of recombinant Mouse IL10RB/IL-10RB. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for IL10RB elisa pair set
Interleukin 10 receptor, beta subunit (IL10RB/IL-10RB) also known as Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, and cytokine receptor family II, member 4, is a subunit for the interleukin-10 receptor. IL10RB/IL-10RB belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins is required for IL10-induced signal transduction. Defects in IL10RB/IL-10RB are the cause of inflammatory bowel disease type 25 (IBD25). It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn's disease and ulcerative colitis phenotypes. Crohn's disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
This IL10RB Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse IL10RB. It contains Mouse IL10RB capture antibody, Mouse IL10RB detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse IL10RB protein.
This IL10RB Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse IL10RB. It contains Mouse IL10RB capture antibody, Mouse IL10RB detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse IL10RB protein.
Product Categories/Family for IL10RB elisa pair set
References
Josephson K, et al. (2001) Crystal structure of the IL-10/IL-10R1 complex reveals a shared receptor binding site. Immunity. 15 (1): 35-46.Yoo KH, et al. (2011) Association of IL10, IL10RA, and IL10RB polymorphisms with benign prostate hyperplasia in Korean population. J Korean Med Sci. 26(5): 659-64.
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Product Notes
The IL10RB (Catalog #AAA8118517) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IL10RB, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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