HE4 ELISA Pair Set | WFDC2 elisa pair set

HE4 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118437

• Synonyms: HE4; HE4 Matched ELISA Antibody Pair Set; Human; Human HE4/WFDC2/WAP5 ELISA Pair Set; WAP four-disulfide core domain 2; dJ461P17.6 Matched ELISA Antibody Pair Set; EDDM4 Matched ELISA Antibody Pair Set; WAP5 Matched ELISA Antibody Pair Set; WFDC2 elisa pair set

• Specificity: Quantitative determination of Human HE4

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 15.625pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human HE4/WFDC2/WAP5 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human HE4/WFDC2/WAP5 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.05ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 50 ng of recombinant Human HE4/WFDC2/WAP5. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for WFDC2 elisa pair set

WAP four-disulfide core domain protein 2, also known as Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2 and HE4, is a secreted protein that contains two WAP domains. WFDC2/HE4 is a member of a family of stable 4-disulfide core proteins that are secreted at high levels. It is expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. It is highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. WFDC2 may be a component of the innate immune defences of the lung, nasal and oral cavities and suggest that WFDC2 functions in concert with related WAP domain containing proteins in epithelial host defence. WFDC2 re-expression in lung carcinomas may prove to be associated with tumour type and should be studied in further detail. Mammary gland expression of tammar WFDC2 during the course of lactation showed WFDC2 was elevated during pregnancy, reduced in early lactation and absent in mid-late lactation. WFDC2/HE4 can undergo a complex series of alternative splicing events that can potentially yield five distinct WAP domain containing protein isoforms.

This HE4 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human HE4. It contains Human HE4 capture antibody, Human HE4 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human HE4 protein.

Product Categories/Family for WFDC2 elisa pair set

Serine Proteases and Regulators

References

Bingle, L. et al., 2002, Oncogene. 21 (17):2768-73. Hellstrom, I. et al., 2003, Cancer Res. 63 (13):3695-700. Bingle, L. et al., 2006, Respir Res. 7 : 61. Galgano, M.T. et al., 2006, Mod Pathol.19 (6):847-53. Sharp, J.A. et al., 2007, Evol Dev. 9 (4): 378-92.