Testing Data
CD44 elisa pair set
CD44 Matched ELISA Antibody Pair Set, Human
Synonyms
CD44; CD44 Matched ELISA Antibody Pair Set; Human; Human CD44 ELISA Pair Set; CD44 molecule (Indian blood group); CDW44 Matched ELISA Antibody Pair Set; CSPG8 Matched ELISA Antibody Pair Set; ECMR-III Matched ELISA Antibody Pair Set; HCELL Matched ELISA Antibody Pair Set; HUTCH-I Matched ELISA Antibody Pair Set; IN Matched ELISA Antibody Pair Set; LHR Matched ELISA Antibody Pair Set; MC56 Matched ELISA Antibody Pair Set; MDU2 Matched ELISA Antibody Pair Set; MDU3 Matched ELISA Antibody Pair Set; MIC4 Matched ELISA Antibody Pair Set; Pgp1 Matched ELISA Antibody Pair Set; CD44 elisa pair set
Specificity
Quantitative determination of Human CD44
Assay Type
Solid Phase Sandwich ELISA
Detection Range
125-8000pg/mL
Sensitivity
125pg/ml
Materials Provided
1. Capture Antibody 0.2 mg/mL of mouse anti-CD44 monoclonal antibody. Dilute to a working concentration of 2ug/mL in CBS before coating.
2. Detection Antibody 0.25 mg/mL mouse anti-CD44 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 190 ng of recombinant CD44. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8ng/mL is recommended.
2. Detection Antibody 0.25 mg/mL mouse anti-CD44 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 190 ng of recombinant CD44. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8ng/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for CD44 elisa pair set
CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis.
This CD44 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CD44. It contains Human CD44 capture antibody, Human CD44 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CD44 protein.
This CD44 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CD44. It contains Human CD44 capture antibody, Human CD44 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CD44 protein.
References
Bajorath J. (2000) Molecular organization, structural features, and ligand binding characteristics of CD44, a highly variable cell surface glycoprotein with multiple functions. Proteins. 39(2): 103-11.Johnson P, et al. (2000) A role for the cell adhesion molecule CD44 and sulfation in leukocyte-endothelial cell adhesion during an inflammatory response? Biochem Pharmacol. 59(5): 455-65.Martin TA, et al. (2003) The role of the CD44/ezrin complex in cancer metastasis. Crit Rev Oncol Hematol. 46(2): 165-86.Toole BP, et al. (2008) Hyaluronan, CD44 and Emmprin: partners in cancer cell chemoresistance. Drug Resist Updat. 11(3): 110-21.Johnson P, et al. (2009) CD44 and its role in inflammation and inflammatory diseases. Inflamm Allergy Drug Targets. 8(3): 208-20.
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Product Notes
The CD44 (Catalog #AAA8118427) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD44, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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