Mouse anti-Human CD44 Monoclonal Antibody | anti-CD44 antibody

Anti-CD44 Antibody (FITC), Mouse Monoclonal

Cat. Num. AAA8106284

• Gene Names: CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD44; Monoclonal Antibody; Anti-CD44 Antibody (FITC); Mouse Monoclonal; CD44 Antibody (FITC); Mouse MAb; CD44 molecule (Indian blood group); Anti-CDW44 Antibody; Anti-CSPG8 Antibody; Anti-ECMR-III Antibody; Anti-HCELL Antibody; Anti-HUTCH-I Antibody; Anti-IN Antibody; Anti-LHR Antibody; Anti-MC56 Antibody; Anti-MDU2 Antibody; Anti-MDU3 Antibody; Anti-MIC4 Antibody; Anti-Pgp1 Antibody; anti-CD44 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 9G5B5
• Specificity: Human CD44
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 1ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CD44 antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human CD44 protein
• Conjugation: FITC
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD44 (rh CD44) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Testing Data

(Profile of peripheral blood lymphocytes analyzed by flow cytometry. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.)

Flow Cytometry (FC/FACS)

(Profile of anti-CD44 reactivity on HeLa cells analyzed by flow cytometry.)

Related Product Information for anti-CD44 antibody

CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis.

References

Bajorath J. (2000) Molecular organization, structural features, and ligand binding characteristics of CD44, a highly variable cell surface glycoprotein with multiple functions. Proteins. 39(2): 103-11.Johnson P, et al. (2000) A role for the cell adhesion molecule CD44 and sulfation in leukocyte-endothelial cell adhesion during an inflammatory response? Biochem Pharmacol. 59(5): 455-65.Martin TA, et al. (2003) The role of the CD44/ezrin complex in cancer metastasis. Crit Rev Oncol Hematol. 46(2): 165-86.Toole BP, et al. (2008) Hyaluronan, CD44 and Emmprin: partners in cancer cell chemoresistance. Drug Resist Updat. 11(3): 110-21.Johnson P, et al. (2009) CD44 and its role in inflammation and inflammatory diseases. Inflamm Allergy Drug Targets. 8(3): 208-20.

NCBI and Uniprot Product Information

• NCBI GI #: 48255935
• NCBI GeneID: 960
• NCBI Accession #: NP_000601.3
• NCBI GenBank Nucleotide #: NM_000610.3
• UniProt Accession #: P16070; O95370; P22511; Q04858; Q13419; Q13957; Q13958; Q13959; A5YRN9; B6EAT9; D3DR12; D3DR13
• Molecular Weight: 81,538 Da
• NCBI Official Full Name: CD44 antigen isoform 1
• NCBI Official Synonym Full Names: CD44 molecule (Indian blood group)
• NCBI Official Symbol: CD44
• NCBI Official Synonym Symbols: IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
• NCBI Protein Information: CD44 antigen; epican; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; heparan sulfate proteoglycan; cell surface glycoprotein CD44; extracellular matrix receptor III; chondroitin sulfate proteoglycan 8; GP90 lymphocyte homing/adhesion rec
• UniProt Protein Name: CD44 antigen
• Protein Family: CD44 antigen
• UniProt Gene Name: CD44
• UniProt Synonym Gene Names: LHR; MDU2; MDU3; MIC4; ECMR-III; PGP-1; PGP-I
• UniProt Entry Name: CD44_HUMAN

NCBI Description

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

CD44: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. 19 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Receptor, misc.

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: Golgi apparatus; focal adhesion; cell surface; basolateral plasma membrane; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: collagen binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; hyaluronic acid binding; hyalurononglucosaminidase activity

Biological Process: extracellular matrix organization and biogenesis; positive regulation of heterotypic cell-cell adhesion; Wnt receptor signaling pathway; glycosaminoglycan metabolic process; cytokine and chemokine mediated signaling pathway; cell-matrix adhesion; pathogenesis; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; hyaluronan catabolic process; extracellular matrix disassembly; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell adhesion; ureteric bud branching; cartilage development; carbohydrate metabolic process; blood coagulation; leukocyte migration; hyaluronan metabolic process; healing during inflammatory response; negative regulation of apoptosis

Disease: Blood Group, Indian System