Testing Data
LILRB3 elisa pair set
LILRB3 Matched ELISA Antibody Pair Set, Human
Synonyms
LILRB3; LILRB3 Matched ELISA Antibody Pair Set; Human; Human LILRB3 ELISA Pair Set; leukocyte immunoglobulin like receptor B3; CD85A Matched ELISA Antibody Pair Set; HL9 Matched ELISA Antibody Pair Set; ILT-5 Matched ELISA Antibody Pair Set; ILT5 Matched ELISA Antibody Pair Set; LILRA6 Matched ELISA Antibody Pair Set; LIR-3 Matched ELISA Antibody Pair Set; LIR3 Matched ELISA Antibody Pair Set; MGC138403 Matched ELISA Antibody Pair Set; PIRB Matched ELISA Antibody Pair Set; XXbac-BCX105G6.7 Matched ELISA Antibody Pair Set; LILRB3 elisa pair set
Specificity
Quantitative determination of Human LILRB3
Assay Type
Solid Phase Sandwich ELISA
Detection Range
78.125-5000pg/mL
Sensitivity
78.125pg/ml
Materials Provided
1. Capture Antibody 0.15 mg/mL of mouse anti-Human LILRB3 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human LILRB3 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.8ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 200 ng of recombinant Human LILRB3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human LILRB3 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.8ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 200 ng of recombinant Human LILRB3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for LILRB3 elisa pair set
Leukocyte immunoglobulin-like receptor subfamily B member 3, also known as Leukocyte immunoglobulin-like receptor 3, Immunoglobulin-like transcript 5, Monocyte inhibitory receptor HL9, CD85 antigen-like family member A, CD85a and LILRB3, is a single-pass type I membrane protein that belongs to the leukocyte receptor cluster (LRC) present on 19q13.4. LILRB3/CD85a contains four Ig-like C2-type (immunoglobulin-like) domains. LILRB3/CD85a contains three copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in the modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. LILRB3/CD85a is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found.
This LILRB3 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human LILRB3. It contains Human LILRB3 capture antibody, Human LILRB3 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human LILRB3 protein.
This LILRB3 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human LILRB3. It contains Human LILRB3 capture antibody, Human LILRB3 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human LILRB3 protein.
Product Categories/Family for LILRB3 elisa pair set
References
Huang, J. et al., 2010, J Virol. 84 (18): 9463-71. Arm J.P. et al., 1997, J. Immunol. 159:2342-2349. Wende H. et al., 2000, Immunogenetics 51:703-713. Yu L.-R. et al., 2007, J. Proteome Res. 6:4150-4162.
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Product Notes
The LILRB3 (Catalog #AAA8118416) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "LILRB3, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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