Factor XIII ELISA Pair Set | F13B elisa pair set

Factor XIII Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118376

• Synonyms: Factor XIII; Factor XIII Matched ELISA Antibody Pair Set; Human; Human Coagulation Factor XIII B chain/F13B ELISA Pair Set; coagulation factor XIII; B polypeptide; Coagulation factor 13 Matched ELISA Antibody Pair Set; Coagulation factor XIII Matched ELISA Antibody Pair Set; FXIIIB Matched ELISA Antibody Pair Set; F13B elisa pair set

• Specificity: Quantitative determination of Human Factor XIII

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 6.25-400pg/mL
• Sensitivity: 6.25pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of rabbit anti-Human Coagulation Factor XIII B chain/F13B monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human Coagulation Factor XIII B chain/F13B polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 21 ng of recombinant Human Coagulation Factor XIII B chain/F13B. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for F13B elisa pair set

Coagulation factor XIII B chain, also known as Fibrin-stabilizing factor B subunit, Protein-glutamine gamma-glutamyltransferase B chain, Transglutaminase B chain and F13B, is a secreted protein which contains 1 Sushi (CCP/SCR) domains. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is composed of just 2 A subunits, which are identical to those of plasma origin. The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin. Factor XIII acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. Defects in F13B are the cause of factor XIII subunit B deficiency (FA13BD) which is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.

This Factor XIII Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Factor XIII. It contains Human Factor XIII capture antibody, Human Factor XIII detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Factor XIII protein.

References

Bottenus R.E., et al., (1990), Nucleotide sequence of the gene for the b subunit of human factor XIII. Biochemistry 29:11195-11209.Ichinose A., et al., (1986), Amino acid sequence of the b subunit of human factor XIII, a protein composed of ten repetitive segments.Biochemistry 25:4633-4638.Ota T., et al., (2004), Complete sequencing and characterization of 21, 243 full-length human cDNAs.Nat. Genet. 36:40-45.