Testing Data
DcR2/TRAIL R4 ELISA Pair Set | TNFRSF10D elisa pair set
DcR2/TRAIL R4 Matched ELISA Antibody Pair Set, Human
Synonyms
DcR2/TRAIL R4; DcR2/TRAIL R4 Matched ELISA Antibody Pair Set; Human; Human TRAIL R4/CD264/TNFRSF10D ELISA Pair Set; tumor necrosis factor receptor superfamily; member 10d; decoy with truncated death domain; CD264 Matched ELISA Antibody Pair Set; DCR2 Matched ELISA Antibody Pair Set; TRAIL-R4 Matched ELISA Antibody Pair Set; TRAILR4 Matched ELISA Antibody Pair Set; TRUNDD Matched ELISA Antibody Pair Set; TNFRSF10D elisa pair set
Specificity
Quantitative determination of Human DcR2/TRAIL R4
Assay Type
Solid Phase Sandwich ELISA
Detection Range
234.38-15000pg/mL
Sensitivity
234.38pg/ml
Materials Provided
1. Capture Antibody 0.3 mg/mL of mouse anti-Human TRAIL R4/CD264/TNFRSF10D monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human TRAIL R4/CD264/TNFRSF10D monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.4ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 345 ng of recombinant Human TRAIL R4/CD264/TNFRSF10D. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 15000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human TRAIL R4/CD264/TNFRSF10D monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.4ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 345 ng of recombinant Human TRAIL R4/CD264/TNFRSF10D. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 15000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for TNFRSF10D elisa pair set
Tumor necrosis factor receptor superfamily member 10D (TNFRSF10D), also known as TNF-related apoptosis-inducing ligand receptor 4 (TRAIL R4), CD264, and Decoy receptor 2, is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis. TRAIL R4/CD264/TNFRSF10D is widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. TRAIL R4/CD264/TNFRSF10D is also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart. The signaling capacity of TRAIL R4 is similar to that of TRAIL R1 and TRAIL R2 with respect to NF-kappaB activation, but differs in its inability to induce apoptosis. TRAIL R4 retains a C-terminal element containing one third of a consensus death domain motif. Transient overexpression of TRAIL R4 in cells normally sensitive to TRAIL-mediated killing confers complete protection, suggesting that one function of TRAIL R4 may be inhibition of TRAIL cytotoxicity.
This DcR2/TRAIL R4 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human DcR2/TRAIL R4. It contains Human DcR2/TRAIL R4 capture antibody, Human DcR2/TRAIL R4 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human DcR2/TRAIL R4 protein.
This DcR2/TRAIL R4 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human DcR2/TRAIL R4. It contains Human DcR2/TRAIL R4 capture antibody, Human DcR2/TRAIL R4 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human DcR2/TRAIL R4 protein.
Product Categories/Family for TNFRSF10D elisa pair set
References
Degli-Esposti MA, et al. (1997) The novel receptor TRAIL-R4 induces NF-kappaB and protects against TRAIL-mediated apoptosis, yet retains an incomplete death domain. Immunity. 7(6): 813-20.Meng RD, et al. (2000) The TRAIL decoy receptor TRUNDD (DcR2, TRAIL-R4) is induced by adenovirus-p53 overexpression and can delay TRAIL-, p53-, and KILLER/DR5-dependent colon cancer apoptosis. Mol Ther. 1(2): 130-44.Bouralexis S, et al. (2003) Progressive resistance of BTK-143 osteosarcoma cells to Apo2L/TRAIL-induced apoptosis is mediated by acquisition of DcR2/TRAIL-R4 expression: resensitisation with chemotherapy. Br J Cancer. 89(1): 206-14.
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Product Notes
The TNFRSF10D (Catalog #AAA8118246) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DcR2/TRAIL R4, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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