Mouse anti-Human PRKRA / PACT Monoclonal Antibody | anti-PRKRA antibody

Mouse Monoclonal [clone 1B9-1A7] (IgG1,k) to Human PRKRA / PACT

Cat. Num. AAA245583

• Gene Names: PRKRA; RAX; PACT; DYT16; HSD14
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
• Purity: Protein A Purified
• Synonyms: PRKRA / PACT; Monoclonal Antibody; Mouse Monoclonal [clone 1B9-1A7] (IgG1; k) to Human PRKRA / PACT; Anti-PRKRA / PACT Antibody (clone 1B9-1A7) IHC-plus; PRKRA; DYT16; HSD14; PKR-associating protein X; PACT; PKR-associated protein X; Human PRKRA; anti-PRKRA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1B9-1A7
• Specificity: Human PACT
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 313

• Applicable Applications for anti-PRKRA antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA, IHC-P (2.5 ug/ml), IP, WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Tissue lysate, Cell lysate, Transfected lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: PRKRA (AAH09470, 1 a.a. ~ 314 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: PRKRA / PACT antibody was raised against pRKRA (AAH09470, 1 a.a. ~ 314 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-PRKRA / PACT antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Western Blot (WB)

(Western blot of PRKRA expression in transfected 293T cell line by PRKRA monoclonal antibody, clone 1B9-1A7.)

Western Blot (WB)

(PRKRA monoclonal antibody, clone 1B9-1A7. Western blot of PRKRA expression in human liver.)

Immunoprecipitation (IP)

(Immunoprecipitation of PRKRA transfected lysate using anti-PRKRA monoclonal antibody and Protein A Magnetic Bead.)

ELISA (EIA)

(Detection limit for recombinant GST tagged PRKRA is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4505581
• NCBI GeneID: 8575
• NCBI Accession #: NP_003681.1
• NCBI GenBank Nucleotide #: NM_003690.4
• UniProt Accession #: O75569; Q53G24; Q6X7T5; Q8NDK4; A8K3I6
• Molecular Weight: 31,635 Da
• NCBI Official Full Name: interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 1
• NCBI Official Synonym Full Names: protein kinase, interferon-inducible double stranded RNA dependent activator
• NCBI Official Symbol: PRKRA
• NCBI Official Synonym Symbols: RAX; PACT; DYT16; HSD14
• NCBI Protein Information: interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; protein activator of the interferon-induced protein kinase; protein kinase, interferon-inducible double stranded RNA-dependent activator; protein kinase, interferon-inducible double-stranded RNA-dependent activator; interferon-inducible double stranded RNA-dependent protein kinase activator A
• UniProt Protein Name: Interferon-inducible double-stranded RNA-dependent protein kinase activator A
• Protein Family: Interferon-inducible double-stranded RNA-dependent protein kinase activator
• UniProt Gene Name: PRKRA
• UniProt Synonym Gene Names: PACT; RAX
• UniProt Entry Name: PRKRA_HUMAN

NCBI Description

This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

PACT: a double strand RNA binding protein (dsRBP). Interacts with TRBP and Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. Activates the interferon-induced protein kinase PKR. Three alternatively spliced human isoforms have been reported.

Protein type: Activator; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: nucleoplasm; membrane; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: protein binding; protein homodimerization activity; enzyme activator activity

Biological Process: positive regulation of catalytic activity; negative regulation of cell proliferation; skeletal morphogenesis; response to virus; outer ear morphogenesis; gene expression; immune response; middle ear morphogenesis; RNA interference, production of siRNA; protein amino acid phosphorylation

Disease: Dystonia 16