Testing Data
MCP-1/CCL2 ELISA Pair Set | CCL2 elisa pair set
MCP-1/CCL2 Matched ELISA Antibody Pair Set, Human
Synonyms
MCP-1/CCL2; MCP-1/CCL2 Matched ELISA Antibody Pair Set; Human; Human MCP-1/CCL2 ELISA Pair Set; chemokine (C-C motif) ligand 2; GDCF-2 Matched ELISA Antibody Pair Set; HC11 Matched ELISA Antibody Pair Set; HSMCR30 Matched ELISA Antibody Pair Set; MCAF Matched ELISA Antibody Pair Set; MCP-1 Matched ELISA Antibody Pair Set; MCP1 Matched ELISA Antibody Pair Set; SCYA2 Matched ELISA Antibody Pair Set; SMC-CF Matched ELISA Antibody Pair Set; CCL2 elisa pair set
Specificity
Quantitative determination of Human MCP-1/CCL2
Assay Type
Solid Phase Sandwich ELISA
Detection Range
3.91-250pg/mL
Sensitivity
3.91pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of rabbit anti-Human MCP-1/CCL2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human MCP-1/CCL2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in Dilution Buffer before use.
3. Standard Each vial contains 14 ng of recombinant Human MCP-1/CCL2. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 250 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human MCP-1/CCL2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in Dilution Buffer before use.
3. Standard Each vial contains 14 ng of recombinant Human MCP-1/CCL2. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 250 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for CCL2 elisa pair set
Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.
This MCP-1/CCL2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human MCP-1/CCL2. It contains Human MCP-1/CCL2 capture antibody, Human MCP-1/CCL2 detector antibody (HRP) and a highly purified Yeast-expressed recombinant Human MCP-1/CCL2 protein.
This MCP-1/CCL2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human MCP-1/CCL2. It contains Human MCP-1/CCL2 capture antibody, Human MCP-1/CCL2 detector antibody (HRP) and a highly purified Yeast-expressed recombinant Human MCP-1/CCL2 protein.
Product Categories/Family for CCL2 elisa pair set
References
Vasquez-Loarte T, Trubnykova M, Guio H. Genetic association meta-analysis: a new classification to assess ethnicity using the association of MCP-1 -2518 polymorphism and tuberculosis susceptibility as a model. BMC Genetics. 2015;16:128.
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Product Notes
The CCL2 (Catalog #AAA8118192) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MCP-1/CCL2, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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