Danio rerio (zebrafish) EFNB2A Cell Lysate | EFNB2A cell lysate
Danio rerio (zebrafish) EFNB2A HEK293 Overexpression Lysate
Gene Names
efnb2a; efnb2; ephrin-B2a; id:ibd5072
Applications
Western Blot
Synonyms
Danio rerio (zebrafish) EFNB2A; Danio rerio (zebrafish) EFNB2A HEK293 Overexpression Lysate; Danio rerio (zebrafish) EFNB2A/Ephrin B2a HEK293 Cell Lysate (WB positive control); Danio rerio (zebrafish) efnb2 Overexpression Lysate; Danio rerio (zebrafish) EFNB2A Overexpression Lysate; Danio rerio (zebrafish) id:ibd5072 Overexpression Lysate; EFNB2A cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
439aa
Sequence
Met1-Ala222
Applicable Applications for EFNB2A cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Danio rerio (zebrafish)
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the zebrafish EFNB2A (O73874) (Met 1-Ala222) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for EFNB2A cell lysate
Efnb2a, also known as ephrin B2a, belongs to the ephrin family. Members of this family are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Efnb2a binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Efnb2a, together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration.
This Danio rerio (zebrafish) EFNB2A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of EFNB2A protein from the overexpression lysate was verified.
This Danio rerio (zebrafish) EFNB2A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of EFNB2A protein from the overexpression lysate was verified.
References
Shaw KM. et al., 2006, Dev Dyn. 235 (7): 1753-60. Kemp HA. et al., 2009, Dev Biol. 327 (2): 313-26. Patten SA. et al., 2012, PLoS One. 7 (2): e31650.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,724 Da
NCBI Official Full Name
ephrin-B2a
NCBI Official Synonym Full Names
ephrin B2a
NCBI Official Symbol
efnb2a
NCBI Official Synonym Symbols
efnb2; ephrin-B2a; id:ibd5072
NCBI Protein Information
ephrin-B2a
UniProt Protein Name
Ephrin-B2a
Protein Family
UniProt Gene Name
efnb2a
UniProt Synonym Gene Names
efnb2
UniProt Entry Name
EFNB2_DANRE
Uniprot Description
Function: Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration
By similarity.
Subunit structure: Binds to the receptor tyrosine kinase ephb4.
Subcellular location: Membrane; Single-pass type I membrane protein.
Post-translational modification: Inducible phosphorylation of tyrosine residues in the cytoplasmic domain
By similarity.
Sequence similarities: Belongs to the ephrin family.Contains 1 ephrin RBD (ephrin receptor-binding) domain.
Research Articles on EFNB2A
Similar Products
Product Notes
The EFNB2A efnb2a (Catalog #AAA8117204) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 439aa. AAA Biotech's Danio rerio (zebrafish) EFNB2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the EFNB2A efnb2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ala22 2. It is sometimes possible for the material contained within the vial of "Danio rerio (zebrafish) EFNB2A, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
