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Typical Testing Data/Standard Curve (for reference only)

Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 ELISA Kit | HSD3B1 elisa kit

Human 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, HSD3B1 ELISA Kit

Gene Names
HSD3B1; I; HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
Reactivity
Human
Synonyms
hydroxy-delta-5-steroid dehydrogenase; 3 beta- and steroid delta-isomerase 1; Human 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; HSD3B1 ELISA Kit; HSD3B1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
No cross-reaction with other factors.
Samples
Serum, plasma or cell culture supernatant and organizations in the natural and recombinant HSD3B1 concentration
Assay Type
Sandwich
Detection Range
20 ng/ml-0.312 ng/ml
Sensitivity
0.06 ng/ml.
Intra-assay Precision
? 8%
Inter-assay Precision
? 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HSD3B1 elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human HSD3B1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,252 Da
NCBI Official Full Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
NCBI Official Synonym Full Names
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
NCBI Official Symbol
HSD3B1
NCBI Official Synonym Symbols
I; HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
NCBI Protein Information
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3-beta-HSD I; progesterone reductase; steroid Delta-isomerase; trophoblast antigen FDO161G; delta-5-3-ketosteroid isomerase; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; short chain dehydrogenase/reductase family 11E, member 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1
UniProt Protein Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
UniProt Gene Name
HSD3B1
UniProt Synonym Gene Names
3BH; HSDB3A; 3-beta-HSD I
UniProt Entry Name
3BHS1_HUMAN

Uniprot Description

HSD3B1: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17- alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone. Belongs to the 3-beta-HSD family.

Protein type: Mitochondrial; EC 5.3.3.1; EC 1.1.1.145; Lipid Metabolism - C21-steroid hormone; Oxidoreductase; Membrane protein, integral; Isomerase; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: endoplasmic reticulum membrane; mitochondrial inner membrane; integral to membrane; mitochondrial intermembrane space; smooth endoplasmic reticulum membrane

Molecular Function: steroid delta-isomerase activity; 3-beta-hydroxy-delta5-steroid dehydrogenase activity

Biological Process: steroid metabolic process; mineralocorticoid biosynthetic process; androgen biosynthetic process; glucocorticoid biosynthetic process; estrogen biosynthetic process; steroid biosynthetic process

Research Articles on HSD3B1

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Product Notes

The Human HSD3B1 hsd3b1 (Catalog #AAA2602869) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2602869 ELISA Kit recognizes Human HSD3B1. It is sometimes possible for the material contained within the vial of "hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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