NAALADL1 cell lysate
Mouse NAALADL1 HEK293 Overexpression Lysate
Gene Names
Naaladl1; Gm964; Naaladasel
Applications
Western Blot
Synonyms
NAALADL1; Mouse NAALADL1 HEK293 Overexpression Lysate; Mouse NAALADL1 HEK293 Cell Lysate (WB positive control); N-acetylated alpha-linked acidic dipeptidase-like 1; Mouse Gm964 Overexpression Lysate; Mouse Naaladasel Overexpression Lysate; NAALADL1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
736aa
Sequence
Pro29-Leu745
Applicable Applications for NAALADL1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the secreted form of mouse NAALADL1 (Q7M758) (Pro 29-Leu 745) was fused with a polyhistidine tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for NAALADL1 cell lysate
N-acetylated-alpha-linked acidic dipeptidase-like protein, also known as NAALADL1, NAALADase L, and Ileal dipeptidyl-peptidase, is a Single-pass type II membrane protein and a member of the peptidase M28 family and M28B subfamily. NAALADase L is mainly expressed in the distal small intestine. It is also expressed in the spleen and testis and Weakly expressed in the brain, locating mainly to the brain stem, amygdala, thalamus, and ventral striatum. NAALADase L is a chloride-activated, membrane-bound, metallopeptidase that cleaves the endogenous neuropeptide N-acetyl-aspartyl-glutamate (NAAG). NAAG acts as a partial NMDA agonist as well as a full agonist at the presynaptic metabotropic glutamate receptor 3 (mGluR3), where it acts to reduce glutamate release. NAALADase L also exhibits a dipeptidyl-peptidase IV type activity. NAALADase inhibition may be a novel therapeutic approach to reduce or inhibit heightened aggressiveness, and possibly to treat aggressive behavior associated with psychiatric disorders.
This Mouse NAALADL1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of NAALADL1 protein from the overexpression lysate was verified.
This Mouse NAALADL1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of NAALADL1 protein from the overexpression lysate was verified.
References
Stauch BL. 1989, Neurosci Lett. 100 (1-3): 295-300. Shneider BL. et al., 1997, J. Biol. Chem. 272: 31006-15. Pangalos MN. et al., 1999, J. Biol. Chem. 274: 8470-83. Thomas AG. et al., 1999, Brain Res. 843 (1-2): 48-52.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
80,513 Da
NCBI Official Full Name
N-acetylated-alpha-linked acidic dipeptidase-like protein
NCBI Official Synonym Full Names
N-acetylated alpha-linked acidic dipeptidase-like 1
NCBI Official Symbol
Naaladl1
NCBI Official Synonym Symbols
Gm964; Naaladasel
NCBI Protein Information
N-acetylated-alpha-linked acidic dipeptidase-like protein; NAALADase L; N-acetylated-alpha-linked acidic dipeptidase like protein
UniProt Protein Name
N-acetylated-alpha-linked acidic dipeptidase-like protein
Protein Family
UniProt Gene Name
Naaladl1
UniProt Synonym Gene Names
Gm964; Naaladasel; NAALADase L
UniProt Entry Name
NALDL_MOUSE
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Product Notes
The NAALADL1 naaladl1 (Catalog #AAA8116939) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 736aa. AAA Biotech's NAALADL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the NAALADL1 naaladl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Pro29-Leu7 45. It is sometimes possible for the material contained within the vial of "NAALADL1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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