SLAMF8 cell lysate
Mouse SLAMF8 HEK293 Overexpression Lysate
Gene Names
Slamf8; Blame; SBBI42; 5830408F06Rik
Applications
Western Blot
Synonyms
SLAMF8; Mouse SLAMF8 HEK293 Overexpression Lysate; Mouse SLAMF8/BLAME HEK293 Cell Lysate (WB positive control); SLAM family member 8; Mouse 5830408F06Rik Overexpression Lysate; Mouse Blame Overexpression Lysate; Mouse SBBI42 Overexpression Lysate; SLAMF8 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
222aa
Sequence
Met1-Asp231
Applicable Applications for SLAMF8 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse SLAMF8 (NP_083360.2) extracellular domain (Met 1-Asp 231) was fused with the a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SLAMF8 cell lysate
The signaling lymphocyte activation molecule (SLAM) family includes homophilic and heterophilic receptors that modulate both adaptive and innate immune responses. These receptors share a common ectodomain organization: a membrane-proximal immunoglobulin constant domain and a membrane-distal immunoglobulin variable domain that is responsible for ligand recognition. SLAM family of receptors is expressed by a wide range of immune cells. Through their cytoplasmic domain, SLAM family receptors associate with SLAM-associated protein (SAP)-related molecules, a group of cytoplasmic adaptors composed almost exclusively of an SRC homology 2 domain. SLAM family receptors, in association with SAP family adaptors, have crucial roles during normal immune reactions in innate and adaptive immune cells.
Mouse SLAM family member 8, also known as B-lymphocyte activator macrophage expressed, BCM-like membrane protein, SLAMF8 and BLAME, is a single-pass type I membrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain. SLAMF8/BLAME is expressed in lymph node, spleen, thymus and bone marrow. It may play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor.
This Mouse SLAMF8 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SLAMF8 protein from the overexpression lysate was verified.
Mouse SLAM family member 8, also known as B-lymphocyte activator macrophage expressed, BCM-like membrane protein, SLAMF8 and BLAME, is a single-pass type I membrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain. SLAMF8/BLAME is expressed in lymph node, spleen, thymus and bone marrow. It may play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor.
This Mouse SLAMF8 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SLAMF8 protein from the overexpression lysate was verified.
Product Categories/Family for SLAMF8 cell lysate
References
Kingsbury G.A., et al., 2001, J. Immunol. 166:5675-80. Zhang Z., et al., 2004, Protein Sci. 13: 2819-24. Veillette, A. et al., 2006, Nat Rev Immunol 6 (1): 56-66. Veillette, A. et al., 2006, Trends Immunol 27 (5): 228-34. Yan, Q. et al., 2007, Proc Natl Acad Sci. USA.104 (25):10583-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,646 Da
NCBI Official Full Name
SLAM family member 8
NCBI Official Synonym Full Names
SLAM family member 8
NCBI Official Symbol
Slamf8
NCBI Official Synonym Symbols
Blame; SBBI42; 5830408F06Rik
NCBI Protein Information
SLAM family member 8; B lymphocyte activator macrophage expressed; B-lymphocyte activator macrophage expressed
UniProt Protein Name
SLAM family member 8
Protein Family
UniProt Gene Name
Slamf8
UniProt Synonym Gene Names
Blame
UniProt Entry Name
SLAF8_MOUSE
Uniprot Description
Function: May play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor. Ref.1
Subcellular location: Membrane; Single-pass type I membrane protein.
Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Research Articles on SLAMF8
Similar Products
Product Notes
The SLAMF8 slamf8 (Catalog #AAA8116742) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 222aa. AAA Biotech's SLAMF8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SLAMF8 slamf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Asp23 1. It is sometimes possible for the material contained within the vial of "SLAMF8, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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