METRN cell lysate
Mouse METRN HEK293 Overexpression Lysate
Gene Names
Metrn; Hyrac; 1810034B16Rik
Applications
Western Blot
Synonyms
METRN; Mouse METRN HEK293 Overexpression Lysate; Mouse Meteorin/METRN HEK293 Cell Lysate (WB positive control); meteorin; glial cell differentiation regulator; Mouse 1810034B16Rik Overexpression Lysate; Mouse Hyrac Overexpression Lysate; METRN cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
526aa
Sequence
Gly22-Asp291
Applicable Applications for METRN cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse METRN (Q8C1Q4-1) (Gly22-Asp291) was expressed with the fused Fc region of human IgG1 at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for METRN cell lysate
METRN (Meteorin, Glial Cell Differentiation Regulator) is a Protein Coding gene. The encoded protein belongs to the meteorin family. It is broadly expressed in the brain, kidney, and other tissues. Meteorin is a novel secreted protein that is expressed in undifferentiated neural progenitors and the astrocyte lineage, including radial glia. It plays important role in the differentiation of glial cells and also in axonal network formation during neurogenesis. Meteorin selectively promoted astrocyte formation from mouse cerebrocortical neurospheres in differentiation culture, whereas it induced cerebellar astrocytes to become radial glia. Meteorin also induced axonal extension in small and intermediate neurons of sensory ganglia by activating nearby satellite glia.
This Mouse METRN overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of METRN protein from the overexpression lysate was verified.
This Mouse METRN overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of METRN protein from the overexpression lysate was verified.
References
Martin J, et al. (2004) The sequence and analysis of duplication-rich human chromosome 16. Nature. 432(7020):988-94. Nishino J, et al. (2004) Meteorin: a secreted protein that regulates glial cell differentiation and promotes axonal extension. EMBO J. 23(9):1998-2008. Ota T, et al. (2004) Complete sequencing and characterization of 21, 243 full-length human cDNAs. Nat Genet. 36(1):40-5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,797 Da
NCBI Official Full Name
meteorin
NCBI Official Synonym Full Names
meteorin, glial cell differentiation regulator
NCBI Official Symbol
Metrn
NCBI Official Synonym Symbols
Hyrac; 1810034B16Rik
NCBI Protein Information
meteorin
UniProt Protein Name
Meteorin
Protein Family
UniProt Gene Name
Metrn
UniProt Synonym Gene Names
Hyrac
UniProt Entry Name
METRN_MOUSE
Uniprot Description
METRN: Involved in both glial cell differentiation and axonal network formation during neurogenesis. Promotes astrocyte differentiation and transforms cerebellar astrocytes into radial glia. Also induces axonal extension in small and intermediate neurons of sensory ganglia by activating nearby satellite glia. Belongs to the meteorin family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space
Biological Process: glial cell differentiation; positive regulation of axonogenesis
Similar Products
Product Notes
The METRN metrn (Catalog #AAA8116735) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 526aa. AAA Biotech's METRN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the METRN metrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Gly22-Asp2 91. It is sometimes possible for the material contained within the vial of "METRN, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
