S100A4 cell lysate
Mouse S100A4 HEK293 Overexpression Lysate
Gene Names
S100a4; 42a; 18A2; Capl; FSp1; Mts1; pk9a; PeL98; metastasin
Applications
Western Blot
Synonyms
S100A4; Mouse S100A4 HEK293 Overexpression Lysate; Mouse S100A4 HEK293 Cell Lysate (WB positive control); S100 calcium binding protein A4; Mouse 18A2 Overexpression Lysate; Mouse 42a Overexpression Lysate; Mouse Capl Overexpression Lysate; Mouse FSp1 Overexpression Lysate; Mouse metastasin Overexpression Lysate; Mouse Mts1 Overexpression Lysate; Mouse PeL98 Overexpression Lysate; Mouse pk9a Overexpression Lysate; S100A4 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
360aa
Sequence
Ala2-Lys101
Applicable Applications for S100A4 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse S100A4 (P07091) (Ala2-Lys101) was expressed with the Fc region of human IgG1 at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for S100A4 cell lysate
S100A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S100 proteins containing two EF-hand calcium-binding motifs. In humans at least 20 S100 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S100A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S100A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin beta, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S100A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.
This Mouse S100A4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of S100A4 protein from the overexpression lysate was verified.
This Mouse S100A4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of S100A4 protein from the overexpression lysate was verified.
Product Categories/Family for S100A4 cell lysate
References
Ambartsumian N. et al., 1995, Gene. 159: 125-30. Marenholz I. et al., 2004, Biochem Biophys Res Commun. 322: 1111-22. Helfman DM. et al., 2005, Br J Cancer. 92: 1955-8. Saleem M. et al., 2006, Proc Natl Acad Sci. 103: 14825-30. Boye K. et al., 2008, Int J Cancer. 123: 1301-10. Garrett SC. et al., 2006, J Biol Chem. 281: 677-80. Kriajevska M. et al., 2002, J Biol Chem. 277: 5299-335.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,721 Da
NCBI Official Full Name
protein S100-A4
NCBI Official Synonym Full Names
S100 calcium binding protein A4
NCBI Official Symbol
S100a4
NCBI Official Synonym Symbols
42a; 18A2; Capl; FSp1; Mts1; pk9a; PeL98; metastasin
NCBI Protein Information
protein S100-A4; S100 calcium-binding protein A4; calvasculin; metastatic cell protein; placental calcium-binding protein; protein 18A2; protein Mts1
UniProt Protein Name
Protein S100-A4
Protein Family
UniProt Gene Name
S100a4
UniProt Synonym Gene Names
Capl; Mts1
UniProt Entry Name
S10A4_MOUSE
Uniprot Description
S100A4: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Protein type: Motility/polarity/chemotaxis
Cellular Component: extracellular space; neuron projection; perinuclear region of cytoplasm; nucleus
Molecular Function: identical protein binding; protein binding; RAGE receptor binding; metal ion binding; calcium ion binding; actin binding; calcium-dependent protein binding
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade
Research Articles on S100A4
Similar Products
Product Notes
The S100A4 s100a4 (Catalog #AAA8116641) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 360aa. AAA Biotech's S100A4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the S100A4 s100a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ala2-Lys10 1. It is sometimes possible for the material contained within the vial of "S100A4, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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