SerpinA10 cell lysate
Mouse SerpinA10 HEK293 Overexpression Lysate
Gene Names
Serpina10; PZI; ZPI
Applications
Western Blot
Synonyms
SerpinA10; Mouse SerpinA10 HEK293 Overexpression Lysate; Mouse SerpinA10/ZPI HEK293 Cell Lysate (WB positive control); serpin peptidase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 10; Mouse PZI Overexpression Lysate; Mouse Serpin A10 Overexpression Lysate; Mouse ZPI Overexpression Lysate; SerpinA10 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
438aa
Sequence
Met1-Leu448
Applicable Applications for SerpinA10 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mature form of mouse SERPINA10 isoform 1 (Q8R121-1) (Met 1-Leu 448) was fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SerpinA10 cell lysate
Protein Z-dependent protease inhibitor, also known as PZ-dependent protease inhibitor, SERPINA1 and ZPI, is a secreted protein that belongs to the serpin family. It is expressed by the liver and secreted in plasma. SERPINA1/Serpin-A1 inhibits factor Xa activity in the presence of protein Z, calcium and phospholipid. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases (serine protease inhibitors). Over 1 serpins have now been identified, these include 36 human proteins, as well as molecules in plants, fungi, bacteria, archaea and certain viruses. Serpins are the largest and most diverse family of protease inhibitors. Most serpins control proteolytic cascades, certain serpins do not inhibit enzymes, but instead perform diverse functions such as storage (ovalbumin, in egg white), hormone carriage proteins (thyroxine-binding globulin, cortisol-binding globulin) and tumor suppressor genes (maspin). Most inhibitory serpins target chymotrypsin-like serine proteases. These enzymes are defined by the presence of a nucleophilic serine residue in their catalytic site. Some serpins inhibit other classes of protease. A number of such serpins have been shown to target cysteine proteases. These enzymes differ from serine proteases in that they are defined by the presence of a nucleophilic cysteine residue, rather than a serine residue, in their catalytic site.
This Mouse SerpinA10 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SerpinA10 protein from the overexpression lysate was verified.
This Mouse SerpinA10 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SerpinA10 protein from the overexpression lysate was verified.
References
Han X, et al., 1998, Proc Natl Acad Sci. USA 95: 9250-5. Han X, et al., 2000, Blood 96: 3049-55. Irving JA, et al., 2000, Genome Res. 10 (12): 1845-64. Irving J, et al., 2002, Mol Biol Evol. 19 (11): 1881-90. Rawlings ND, et al., 2004, Biochem J. 378 (Pt 3): 705-16. Water N, et al., 2004, Br J Haematol. 127:190-4. Wei Z, et al., 2009, Blood 114 (17): 3662-7. Whisstock JC, et al., 2010, J Biol Chem. 285 (32): 24307-12.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
160,000
NCBI Official Full Name
protein Z-dependent protease inhibitor
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
NCBI Official Symbol
Serpina10
NCBI Official Synonym Symbols
PZI; ZPI
NCBI Protein Information
protein Z-dependent protease inhibitor; serpin A10; PZ-dependent protease inhibitor; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
UniProt Protein Name
Protein Z-dependent protease inhibitor
Protein Family
UniProt Gene Name
Serpina10
UniProt Synonym Gene Names
Zpi; PZ-dependent protease inhibitor; PZI
UniProt Entry Name
ZPI_MOUSE
NCBI Description
The protein encoded by this gene is a member of the large serpin family of proteins, and is also known as serpin PZ-dependent protease inhibitor (ZPI or PZI). This protein is thought to play an important role in the regulation of coagulation. It directly inhibits factor XIa, and also inhibits factor Xa in the presence of calcium, phospholipids, and protein Z (PZ). Deficiencies in this gene lead to an increase in thrombosis. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms. [provided by RefSeq, Aug 2014]
Uniprot Description
SERPINA10: Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Belongs to the serpin family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; protease inhibitor activity
Biological Process: hemostasis; negative regulation of peptidase activity; blood coagulation
Research Articles on SerpinA10
Similar Products
Product Notes
The SerpinA10 serpina10 (Catalog #AAA8116532) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 438aa. AAA Biotech's SerpinA10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SerpinA10 serpina10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Leu44 8. It is sometimes possible for the material contained within the vial of "SerpinA10, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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