Rabbit Cartilage Intermediate Layer Protein (CILP) Polyclonal Antibody | anti-CILP antibody

Polyclonal Antibody to Cartilage Intermediate Layer Protein (CILP)

Cat. Num. AAA2006189

• Gene Names: CILP; CILP-1; HsT18872
• Reactivity: Homo sapiens (Human)
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Cartilage Intermediate Layer Protein (CILP); Polyclonal Antibody; Polyclonal Antibody to Cartilage Intermediate Layer Protein (CILP); anti-CILP antibody

• Host: Rabbit
• Reactivity: Homo sapiens (Human)
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 0.23mg/ml (varies by lot)

• Applicable Applications for anti-CILP antibody: Western Blot (WB); Immunohistochemistry (IHC) - Formalin/Paraffin; Immunocytochemistry (ICC); Immunoprecipitation (IP).
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Immunogen: Recombinant CILP (Ile603~Ala846) expressed in E.coli ( MBS2009475 ).
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Recombinant CILP, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Liver cancer Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human CILP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Stomach Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human CILP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

NCBI and Uniprot Product Information

• NCBI GI #: 192449445
• NCBI GeneID: 8483
• NCBI Accession #: NP_003604.3
• NCBI GenBank Nucleotide #: NM_003613.3
• UniProt Accession #: O75339; Q6UW99; Q8IYI5; B2R8F7
• NCBI Official Full Name: cartilage intermediate layer protein 1 preproprotein
• NCBI Official Synonym Full Names: cartilage intermediate layer protein
• NCBI Official Symbol: CILP
• NCBI Official Synonym Symbols: CILP-1; HsT18872
• NCBI Protein Information: cartilage intermediate layer protein 1
• UniProt Protein Name: Cartilage intermediate layer protein 1
• Protein Family: Cartilage intermediate layer protein
• UniProt Gene Name: CILP
• UniProt Synonym Gene Names: CILP-1

NCBI Description

Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010]

Uniprot Description

CILP: Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Defects in CILP are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, is conferred by variant p.Ile395Thr (PubMed:15864306).

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix

Molecular Function: alkaline phosphatase activity; nucleotide diphosphatase activity

Biological Process: negative regulation of insulin-like growth factor receptor signaling pathway

Disease: Intervertebral Disc Disease