SerpinA10 / ZPI Recombinant Protein | ZPI recombinant protein

Recombinant Mouse SerpinA10 / ZPI Protein (His tag)

Cat. Num. AAA2547042

• Gene Names: Serpina10; PZI; ZPI
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: SerpinA10 / ZPI; Recombinant Mouse SerpinA10 / ZPI Protein (His tag); MGC25863; PZI; ZPI; ZPI recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 394

• Application Notes: The secreted recombinant mouse SERPINA10 comprises 438 amino acids with a predicted molecular mass of 51 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 60-80 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Phe 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ZPI recombinant protein

Background: Mouse protein Z-dependent protease inhibitor, also known as PZ-dependent protease inhibitor, SERPINA10 and ZPI, is a secreted protein which belongs to the serpin family. It is expressed by the liver and secreted in plasma. SERPINA10 / Serpin-A10 inhibits factor Xa activity in the presence of protein Z, calcium and phospholipid. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases (serine protease inhibitors).Over 1000 serpins have now been identified, these include 36 human proteins, as well as molecules in plants, fungi, bacteria, archaea and certain viruses. Serpins are the largest and most diverse family of protease inhibitors. Most serpins control proteolytic cascades, certain serpins do not inhibit enzymes, but instead perform diverse functions such as storage (ovalbumin, in egg white), hormone carriage proteins (thyroxine-binding globulin, cortisol-binding globulin) and tumor suppressor genes (maspin). Most inhibitory serpins target chymotrypsin-like serine proteases. These enzymes are defined by the presence of a nucleophilic serine residue in their catalytic site. Some serpins inhibit other classes of protease. A number of such serpins have been shown to target cysteine proteases. These enzymes differ from serine proteases in that they are defined by the presence of a nucleophilic cysteine residue, rather than a serine residue, in their catalytic site.

Description: A DNA sequence encoding the mature form of mouse SERPINA10 isoform 1 (Q8R121-1) (Met 1-Leu 448) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 673130998
• NCBI GeneID: 217847
• NCBI Accession #: NP_001288333.1
• NCBI GenBank Nucleotide #: NM_001301404.1
• UniProt Accession #: Q8R121; Q8VCV8
• Molecular Weight: 45,298 Da
• NCBI Official Full Name: protein Z-dependent protease inhibitor isoform 2
• NCBI Official Synonym Full Names: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
• NCBI Official Symbol: Serpina10
• NCBI Official Synonym Symbols: PZI; ZPI
• NCBI Protein Information: protein Z-dependent protease inhibitor
• UniProt Protein Name: Protein Z-dependent protease inhibitor
• UniProt Gene Name: Serpina10
• UniProt Synonym Gene Names: Zpi; PZ-dependent protease inhibitor; PZI

NCBI Description

The protein encoded by this gene is a member of the large serpin family of proteins, and is also known as serpin PZ-dependent protease inhibitor (ZPI or PZI). This protein is thought to play an important role in the regulation of coagulation. It directly inhibits factor XIa, and also inhibits factor Xa in the presence of calcium, phospholipids, and protein Z (PZ). Deficiencies in this gene lead to an increase in thrombosis. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms. [provided by RefSeq, Aug 2014]

Uniprot Description

SERPINA10: Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12|12 E

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity