SerpinB8 cell lysate
Mouse SerpinB8 Baculovirus-Insect cells Overexpression Lysate
Gene Names
Serpinb8; CAP2; NK10; Spi8; CAP-2; ovalbumin
Applications
Western Blot
Synonyms
SerpinB8; Mouse SerpinB8 Baculovirus-Insect cells Overexpression Lysate; Mouse SerpinB8 Insect Cell Lysate (WB positive control); serpin peptidase inhibitor; clade B (ovalbumin); member 8; Mouse CAP-2 Overexpression Lysate; Mouse CAP2 Overexpression Lysate; Mouse NK10 Overexpression Lysate; Mouse ovalbumin Overexpression Lysate; Mouse Spi8 Overexpression Lysate; SerpinB8 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
385aa
Sequence
Met1-Pro374
Applicable Applications for SerpinB8 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse SERPINB8 (NP_035589.1) (Met 1-Pro 374) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SerpinB8 cell lysate
Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner.
Mouse SerpinB8, also known as Cytoplasmic antiproteinase 2, Peptidase inhibitor 8, SerpinB8, PI-8, SERPINB8 and CAP2, is a member of the Serpin superfamily. SERPINB8 was broadly expressed. In normal neuroendocrine tissues, strongest SerpinB8 expression was detected in islets of Langerhans of the pancreas. Moderate SerpinB8 expression was observed in neuroendocrine cells of the thyroid, adrenal cortex, colon, and pituitary gland. In the pancreas, SerpinB8 is specifically expressed by insulin-producing beta cells, and can be used as an additional immunohistochemical marker. Mouse SerpinB8 distribution alters during kidney regeneration, possibly to control a prohormone convertase involved in inflammation or tissue repair.
This Mouse SerpinB8 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of SerpinB8 protein from the overexpression lysate was verified.
Mouse SerpinB8, also known as Cytoplasmic antiproteinase 2, Peptidase inhibitor 8, SerpinB8, PI-8, SERPINB8 and CAP2, is a member of the Serpin superfamily. SERPINB8 was broadly expressed. In normal neuroendocrine tissues, strongest SerpinB8 expression was detected in islets of Langerhans of the pancreas. Moderate SerpinB8 expression was observed in neuroendocrine cells of the thyroid, adrenal cortex, colon, and pituitary gland. In the pancreas, SerpinB8 is specifically expressed by insulin-producing beta cells, and can be used as an additional immunohistochemical marker. Mouse SerpinB8 distribution alters during kidney regeneration, possibly to control a prohormone convertase involved in inflammation or tissue repair.
This Mouse SerpinB8 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of SerpinB8 protein from the overexpression lysate was verified.
References
Sumi, Y. et al., 1989, J. Biochem. 106: 703-7. Rawlings, N.D. et al., 2004, Biochem J. 378: 705-16. Gillard, A. et al., 2006, Am J Nephrol. 26 (1): 34-42. Filleur, S. et al., 2009, J Cell Biochem. 106 (5): 769-75. de Koning, P.J. et al., 2009, Pancreas. 38 (4): 461-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,150 Da
NCBI Official Full Name
serpin B8 isoform 1
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade B, member 8
NCBI Official Symbol
Serpinb8
NCBI Official Synonym Symbols
CAP2; NK10; Spi8; CAP-2; ovalbumin
NCBI Protein Information
serpin B8; serine protease inhibitor 8; serine (or cysteine) peptdiase inhibitor, clade B, member 8; serine (or cysteine) proteinase inhibitor, clade B, member 8
UniProt Protein Name
Serpin B8
Protein Family
UniProt Gene Name
Serpinb8
UniProt Entry Name
SPB8_MOUSE
Uniprot Description
SERPINB8: Belongs to the serpin family. Ov-serpin subfamily. 2 isoforms of the human protein are produced by alternative splicing
Protein type: Inhibitor
Cellular Component: extracellular space; cytoplasm; cytosol
Molecular Function: serine-type endopeptidase inhibitor activity; protease inhibitor activity
Biological Process: negative regulation of peptidase activity
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Product Notes
The SerpinB8 serpinb8 (Catalog #AAA8116468) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 385aa. AAA Biotech's SerpinB8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SerpinB8 serpinb8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro37 4. It is sometimes possible for the material contained within the vial of "SerpinB8, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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