Alpha 2 Antiplasmin/SerpinF2 Cell Lysate | SerpinF2 cell lysate
Mouse Alpha 2 Antiplasmin/SerpinF2 HEK293 Overexpression Lysate
Gene Names
Serpinf2; Pli; AI747498; Serpimf2
Applications
Western Blot
Synonyms
Alpha 2 Antiplasmin/SerpinF2; Mouse Alpha 2 Antiplasmin/SerpinF2 HEK293 Overexpression Lysate; Mouse SerpinF2 HEK293 Cell Lysate (WB positive control); serpin peptidase inhibitor; clade F; member 2; Mouse AI747498 Overexpression Lysate; Mouse Pli Overexpression Lysate; Mouse Serpimf2 Overexpression Lysate; SerpinF2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
475aa
Sequence
Met1-Lys491
Applicable Applications for SerpinF2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse Serpin F2 (NP_032904.1) (Met 1-Lys 491) was expressed with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SerpinF2 cell lysate
SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily. SerpinF2 is the principal physiological inhibitor of serine protease plasmin, and as well as, an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed in muscle, intestine, central nervous system, and placenta at a moderate level. It is indicated that Serpin F2 is a key regulator of plasmin-mediated proteolysis in these tissues. Alpha-2 AP is an unusual serpin in that it contains extensive N-and C-terminal sequences flanking the serpin domain. The N-terminal sequence is crosslinked to fibrin by factor XIIIa, whereas the C-terminal region mediates the initial interaction with plasmin. SerpinF2 is one of the inhibitors of fibrinolysis, which acts as the primary inhibitor of plasmin(ogen). It is a specific plasmin inhibitor, and is important in modulating the effectiveness and persistence of fibrin with respect to its susceptibility to digestion and removal by plasmin. Alpha-2 AP plays the dominant role in inhibiting both plasma clot lysis and thrombus lysis, and accordingly, the congenital deficiency of Alpha-2 antiplasmin causes a rare bleeding disorder because of increased fibrinolysis. Thus, it may be a useful target for developing more effective treatment of thrombotic diseases.
This Mouse Alpha 2 Antiplasmin/SerpinF2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Alpha 2 Antiplasmin/SerpinF2 protein from the overexpression lysate was verified.
This Mouse Alpha 2 Antiplasmin/SerpinF2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Alpha 2 Antiplasmin/SerpinF2 protein from the overexpression lysate was verified.
References
Lee KN, et al. (2004) Alpha2-antiplasmin: potential therapeutic roles in fibrin survival and removal. Curr Med Chem Cardiovasc Hematol Agents. 2(4): 303-10.Matsuno H. (2006) Alpha2-antiplasmin on cardiovascular diseases. Curr Pharm Des. 12(7): 841-7.Burnouf T, et al. (2007) Impact of Triton X-100 on alpha 2-antiplasmin (SERPINF2) activity in solvent/detergent-treated plasma. Biologicals. 35(4): 349-53.Carpenter SL, et al. (2008) Alpha2-antiplasmin and its deficiency: fibrinolysis out of balance. Haemophilia. 14(6): 1250-4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
160,000
NCBI Official Full Name
alpha-2-antiplasmin
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade F, member 2
NCBI Official Symbol
Serpinf2
NCBI Official Synonym Symbols
Pli; AI747498; Serpimf2
NCBI Protein Information
alpha-2-antiplasmin; serpin F2; alpha-2-AP; alpha-2-PI; alpha 2 antiplasmin; alpha-2-plasmin inhibitor; plasmin inhibitor alpha 2; pigment epithelium derived factor; serine (or cysteine) proteinase inhibitor, clade F, member 2
UniProt Protein Name
Alpha-2-antiplasmin
Protein Family
UniProt Gene Name
Serpinf2
UniProt Synonym Gene Names
Pli; Alpha-2-AP; Alpha-2-PI
UniProt Entry Name
A2AP_MOUSE
Uniprot Description
SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; protein homodimerization activity; protease binding; protease inhibitor activity
Biological Process: negative regulation of fibrinolysis; collagen fibril organization; renin-angiotensin regulation of blood vessel size; positive regulation of smooth muscle cell proliferation; positive regulation of stress fiber formation; positive regulation of collagen biosynthetic process; acute-phase response; positive regulation of JNK cascade; negative regulation of peptidase activity; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation
Disease: Alpha-2-plasmin Inhibitor Deficiency
Research Articles on SerpinF2
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Product Notes
The SerpinF2 serpinf2 (Catalog #AAA8116431) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 475aa. AAA Biotech's Alpha 2 Antiplasmin/SerpinF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SerpinF2 serpinf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Lys49 1. It is sometimes possible for the material contained within the vial of "Alpha 2 Antiplasmin/SerpinF2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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