LILRB1 cell lysate
Human LILRB1 HEK293 Overexpression Lysate
Gene Names
LILRB1; ILT2; LIR1; MIR7; PIRB; CD85J; ILT-2; LIR-1; MIR-7; PIR-B
Applications
Western Blot
Synonyms
LILRB1; Human LILRB1 HEK293 Overexpression Lysate; Human LILRB1/CD85j/ILT2 HEK293 Cell Lysate (WB positive control); leukocyte immunoglobulin like receptor B1; Human CD85j Overexpression Lysate; Human ILT-2 Overexpression Lysate; Human ILT2 Overexpression Lysate; Human LIR-1 Overexpression Lysate; Human LIR1 Overexpression Lysate; Human MIR-7 Overexpression Lysate; Human MIR7 Overexpression Lysate; Human XXbac-BCX85G21.4 Overexpression Lysate; LILRB1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
676aa
Sequence
Met1-His458
Applicable Applications for LILRB1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human LILRB1 (ADJ55949.1) (Met1-His458) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for LILRB1 cell lysate
LILRB1 (Leukocyte Immunoglobulin Like Receptor B1) is a Protein Coding gene. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). LILRB1 is encoded within the leukocyte receptor complex on 19q13.4, previously implicated to be a susceptibility region to systemic lupus erythematosus (SLE). LILRB1 binds to a wide range of Major Histocompatibility Complex Class I molecules (MHCIs) and transduces negative signals that can, for example, prevent the killing of MHCI-expressing cells. LILRB1 transcription is correlated with surface protein expression on NK cells. Diseases associated with LILRB1 include Alzheimer's Disease 12 and Severe Pre-Eclampsia.
This Human LILRB1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LILRB1 protein from the overexpression lysate was verified.
This Human LILRB1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LILRB1 protein from the overexpression lysate was verified.
Product Categories/Family for LILRB1 cell lysate
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
49,356 Da
NCBI Official Full Name
LIR-1
NCBI Official Synonym Full Names
leukocyte immunoglobulin like receptor B1
NCBI Official Symbol
LILRB1
NCBI Official Synonym Symbols
ILT2; LIR1; MIR7; PIRB; CD85J; ILT-2; LIR-1; MIR-7; PIR-B
NCBI Protein Information
leukocyte immunoglobulin-like receptor subfamily B member 1
Protein Family
NCBI Description
This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Research Articles on LILRB1
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Product Notes
The LILRB1 (Catalog #AAA8115601) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 676aa. AAA Biotech's LILRB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the LILRB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-His45 8. It is sometimes possible for the material contained within the vial of "LILRB1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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