MFAP5 cell lysate
Human MFAP5 HEK293 Overexpression Lysate
Gene Names
MFAP5; MP25; MAGP2
Applications
Western Blot
Synonyms
MFAP5; Human MFAP5 HEK293 Overexpression Lysate; Human MFAP5/MAGP2 HEK293 Cell Lysate (WB positive control); microfibrillar associated protein 5; Human AAT9 Overexpression Lysate; Human MAGP-2 Overexpression Lysate; Human MAGP2 Overexpression Lysate; Human MFAP-5 Overexpression Lysate; Human MP25 Overexpression Lysate; MFAP5 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
163aa
Sequence
Met1-Leu173
Applicable Applications for MFAP5 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human MFAP5 (Q13361) (Met1-Leu173) with a C-terminal polyhistidine tag was expressed.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for MFAP5 cell lysate
MFAP5 (Microfibril Associated Protein 5, also known as MAGP2) is a Protein Coding gene. MFAP5 is a component of the elastin-associated microfibrils. It belongs to the MFAP family. As a 25-kD microfibril-associated glycoprotein, MFAP5 is rich in serine and threonine residues. It stimulates the assembly of elastic fibers, a complex structure composed of a tropoelastin inner core and microfibril outer mantle comprising proteins such as fibrillins and microfibril-associated glycoproteins that guide tropoelastin deposition. MFAP5 also stabilizes type 1 procollagen and thus plays an important role in extracellular matrix homeostasis. It has multiple binding regions on fibrillins and has a covalent periodic association with fibrillin-containing microfibrils. Diseases associated with MFAP5 include Aortic Aneurysm, Familial Thoracic 9, and Familial Thoracic Aortic Aneurysm And Aortic Dissection.
This Human MFAP5 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of MFAP5 protein from the overexpression lysate was verified.
This Human MFAP5 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of MFAP5 protein from the overexpression lysate was verified.
References
Hatzinikolas G, et al. (1998) The exon structure of the human MAGP-2 gene. Similarity with the MAGP-1 gene is confined to two exons encoding a cysteine-rich region. J Biol Chem. 273(45):29309-14.Gibson MA, et al. (1996) Further characterization of proteins associated with elastic fiber microfibrils including the molecular cloning of MAGP-2 (MP25). J Biol Chem. 271(2): 1096-103.Miyamoto A, et al. (2006) Microfibrillar proteins MAGP-1 and MAGP-2 induce Notch1 extracellular domain dissociation and receptor activation. J Biol Chem. 281(15):10089-97.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,612 Da
NCBI Official Full Name
microfibrillar-associated protein 5
NCBI Official Synonym Full Names
microfibrillar associated protein 5
NCBI Official Symbol
MFAP5
NCBI Official Synonym Symbols
MP25; MAGP2
NCBI Protein Information
microfibrillar-associated protein 5; MAGP-2; MFAP-5; microfibril-associated glycoprotein 2; microfibril-associated glycoprotein-2
UniProt Protein Name
Microfibrillar-associated protein 5
Protein Family
UniProt Gene Name
MFAP5
UniProt Synonym Gene Names
MAGP2; MFAP-5; MAGP-2
UniProt Entry Name
MFAP5_HUMAN
NCBI Description
This gene encodes a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. It lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this encoded protein has some functions that are distinct from those of MFAP2. [provided by RefSeq, Jul 2008]
Uniprot Description
MFAP5: Component of the elastin-associated microfibrils. Belongs to the MFAP family.
Protein type: Secreted, signal peptide; Secreted; Extracellular matrix
Chromosomal Location of Human Ortholog: 12p13.1-p12.3
Cellular Component: microfibril; extracellular region
Molecular Function: extracellular matrix structural constituent
Biological Process: extracellular matrix organization and biogenesis; fibril organization and biogenesis
Disease: Aortic Aneurysm, Familial Thoracic 9
Research Articles on MFAP5
Similar Products
Product Notes
The MFAP5 mfap5 (Catalog #AAA8114981) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 163aa. AAA Biotech's MFAP5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the MFAP5 mfap5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Leu17 3. It is sometimes possible for the material contained within the vial of "MFAP5, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
