COX-2 Cell Lysate | PTGS2 cell lysate

Human COX-2 Baculovirus-Insect cells Overexpression Lysate

Gene Names

PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS

Applications

Western Blot

Synonyms

COX-2; Human COX-2 Baculovirus-Insect cells Overexpression Lysate; Human PTGS2/COX2/PGHS-2 Insect Cell Lysate (WB positive control); prostaglandin-endoperoxide synthase 2; Human COX-2 Overexpression Lysate; Human COX2 Overexpression Lysate; Human GRIPGHS Overexpression Lysate; Human hCox-2 Overexpression Lysate; Human PGG/HS Overexpression Lysate; Human PGHS-2 Overexpression Lysate; Human PHS-2 Overexpression Lysate; PTGS2 cell lysate

Ordering
Cat. Num. AAA8114871 For Research Use Only!

Host

Baculovirus-Insect cells

Form/Format

1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Sequence Positions

597aa

Sequence

Met1-Leu604

Applicable Applications for PTGS2 cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the human PTGS2 (NP_000954.1) (Met 1-Leu 604) was fused with a polyhistidine tag at the C-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.

Related Product Information for PTGS2 cell lysate

PTGS2, also known as COX-2, is s component of Prostaglandin-endoperoxide synthase (PTGS). PTGS, also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. PTGS2 is overexpressed in many cancers. The overexpression of PTGS2 along with increased angiogenesis and GLUT-1 expression is significantly associated with gallbladder carcinomas. Furthermore the product of COX-2, PGH2 is converted by prostaglandin E2 synthase into PGE2, which in turn can stimulate cancer progression. Consequently inhibiting COX-2 may have benefit in the prevention and treatment of these types of cancer. PTGS2 is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. It mediates the formation of prostaglandins from arachidonate and may have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.

This Human COX-2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of COX-2 protein from the overexpression lysate was verified.

Product Categories/Family for PTGS2 cell lysate

Neuroinflammation Cancer Drug Targets; IL17 signaling pathway

References

Picot, et al. (1994) The X-ray crystal structure of the membrane protein prostaglandin H2 synthase-1. Nature. 367(6460):243-9.
Xie W, et al. (1991) Expression of a Mitogen-Responsive Gene Encoding Prostaglandin Synthase is Regulated by mRNA Splicing. Proceedings of the National Academy of Sciences. 88(7):2692-6.
Hla T, et al. (1992) Human Cyclooxygenase-2 cDNA. Proceedings of the National Academy of Sciences. 89(16):7384-8.

NCBI and Uniprot Product Information

NCBI GI #

4506265

NCBI GeneID

5743

NCBI Accession #

NP_000954.1

NCBI GenBank Nucleotide #

NM_000963.2

UniProt Accession #

P35354; Q16876; A8K802

Molecular Weight

68,996 Da

NCBI Official Full Name

prostaglandin G/H synthase 2

NCBI Official Synonym Full Names

prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

NCBI Official Symbol

PTGS2

NCBI Official Synonym Symbols

COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS

NCBI Protein Information

prostaglandin G/H synthase 2; PHS II; PGH synthase 2; cyclooxygenase 2b; prostaglandin H2 synthase 2

UniProt Protein Name

Prostaglandin G/H synthase 2

Protein Family

Prostaglandin G/H synthase

UniProt Gene Name

PTGS2

UniProt Synonym Gene Names

COX2; COX-2; PGH synthase 2; PGHS-2

UniProt Entry Name

PGH2_HUMAN

NCBI Description

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

Uniprot Description

COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family.

Protein type: Lipid Metabolism - arachidonic acid; EC 1.14.99.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 1q25.2-q25.3

Cellular Component: endoplasmic reticulum membrane; neuron projection; protein complex; cytoplasm; caveola; nucleus

Molecular Function: prostaglandin-endoperoxide synthase activity; protein homodimerization activity; peroxidase activity; enzyme binding; arachidonate 15-lipoxygenase activity; metal ion binding; heme binding; lipid binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; positive regulation of smooth muscle cell proliferation; response to lithium ion; response to glucocorticoid stimulus; positive regulation of NF-kappaB import into nucleus; response to lipopolysaccharide; decidualization; sensory perception of pain; prostaglandin biosynthetic process; negative regulation of synaptic transmission, dopaminergic; positive regulation of synaptic transmission, glutamatergic; bone mineralization; response to estradiol stimulus; negative regulation of cell cycle; negative regulation of cell proliferation; response to vitamin D; lipoxygenase pathway; nicotinamide metabolic process; negative regulation of smooth muscle contraction; regulation of blood pressure; arachidonic acid metabolic process; angiogenesis; positive regulation of smooth muscle contraction; inflammatory response; response to fructose stimulus; water-soluble vitamin metabolic process; positive regulation of prostaglandin biosynthetic process; response to drug; negative regulation of calcium ion transport; vitamin metabolic process; cyclooxygenase pathway; positive regulation of synaptic plasticity; learning; memory; prostaglandin metabolic process; hair cycle; response to manganese ion; ovulation; NAD metabolic process; regulation of inflammatory response; brown fat cell differentiation; positive regulation of fever; positive regulation of vasoconstriction; response to oxidative stress; cell motility; embryo implantation

Research Articles on PTGS2

1. The cytoplasmic COX-2 expression were observed in 74.3% and 68.6% of cases respectively.

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Product Notes
The PTGS2 ptgs2 (Catalog #AAA8114871) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 597aa. AAA Biotech's COX-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the PTGS2 ptgs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Leu60 4. It is sometimes possible for the material contained within the vial of "COX-2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.