CD300C cell lysate
Human CD300C HEK293 Overexpression Lysate
Gene Names
CD300C; LIR; CLM-6; CMRF35; IGSF16; CMRF-35; CMRF35A; CMRF-35A; CMRF35A1; CMRF35-A1
Applications
Western Blot
Synonyms
CD300C; Human CD300C HEK293 Overexpression Lysate; Human CD300C/CMRF-35/IGSF16 HEK293 Cell Lysate (WB positive control); CD300c molecule; Human CLM-6 Overexpression Lysate; Human CMRF-35 Overexpression Lysate; Human CMRF-35A Overexpression Lysate; Human CMRF35 Overexpression Lysate; Human CMRF35-A1 Overexpression Lysate; Human CMRF35A Overexpression Lysate; Human CMRF35A1 Overexpression Lysate; Human IGSF16 Overexpression Lysate; Human LIR Overexpression Lysate; CD300C cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
166aa
Sequence
Met1-Arg183
Applicable Applications for CD300C cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human CD300C (NP_006669.1) extracellular domain (Met 1-Arg 183) was expressed, with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CD300C cell lysate
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD300 is a glycoprotein family of cell surface molecules that regulate a diverse array of cellular processes via their triggering and inhibitory receptor functions. The CD300 family of myeloid immunoglobulin receptors includes activating(CD300b, CD300e) and inhibitory members(CD300a, CD300f), as well as CD300c and CD300d, whose function is uncertain.
This Human CD300C overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD300C protein from the overexpression lysate was verified.
This Human CD300C overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD300C protein from the overexpression lysate was verified.
Product Categories/Family for CD300C cell lysate
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Lankry D, et al. (2010) Expression and function of CD300 in NK cells.185(5): 2877-86.Agueda MB, et al. (2010) CD300 heterocomplexes, a new and family-restricted mechanism for myeloid cell signaling regulation. Journal of biological chemistry. 285: 41781-94.Clark GJ, et al. (2009) The CD300 molecules regulate monocyte and dendritic cell functions. 214 (9-10): 730-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,830 Da
NCBI Official Full Name
CMRF35-like molecule 6
NCBI Official Synonym Full Names
CD300c molecule
NCBI Official Symbol
CD300C
NCBI Official Synonym Symbols
LIR; CLM-6; CMRF35; IGSF16; CMRF-35; CMRF35A; CMRF-35A; CMRF35A1; CMRF35-A1
NCBI Protein Information
CMRF35-like molecule 6; CD300 antigen-like family member C; CD300c antigen; CMRF35 antigen; CMRF35 leukocyte immunoglobulin-like receptor; CMRF35A leukocyte immunoglobulin-like receptor; immunoglobulin superfamily member 16
UniProt Protein Name
CMRF35-like molecule 6
Protein Family
UniProt Gene Name
CD300C
UniProt Synonym Gene Names
CMRF35; CMRF35A; CMRF35A1; IGSF16; CLM-6; CMRF-35; IgSF16
UniProt Entry Name
CLM6_HUMAN
NCBI Description
The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes (Jackson et al., 1992 [PubMed 1349532]).[supplied by OMIM, Mar 2008]
Uniprot Description
CD300C: Belongs to the CD300 family
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: integral to plasma membrane
Molecular Function: transmembrane receptor activity
Biological Process: immune system process; cellular defense response; signal transduction
Research Articles on CD300C
Similar Products
Product Notes
The CD300C cd300c (Catalog #AAA8114793) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 166aa. AAA Biotech's CD300C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CD300C cd300c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Arg18 3. It is sometimes possible for the material contained within the vial of "CD300C, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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