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TRIB2 cell lysate

Human TRIB2 Baculovirus-Insect cells Overexpression Lysate

Gene Names
TRIB2; C5FW; TRB2; GS3955
Applications
Western Blot
Synonyms
TRIB2; Human TRIB2 Baculovirus-Insect cells Overexpression Lysate; Human TRIB2/TRB2 Insect Cell Lysate (WB positive control); tribbles pseudokinase 2; Human C5FW Overexpression Lysate; Human FLJ57420 Overexpression Lysate; Human GS3955 Overexpression Lysate; Human TRB2 Overexpression Lysate; Human TRIB2 Overexpression Lysate; TRIB2 cell lysate
Ordering
For Research Use Only!
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
580aa
Sequence
Met1-Asn343
Applicable Applications for TRIB2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human TRIB2 (NP_067675.1) (Met 1-Asn 343) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for TRIB2 cell lysate
Tribbles homolog 2, also known as TRB-2, and Trib2, is a member of the protein kinase superfamily and Tribbles subfamily (Trib1, Trib2, Trib3). The identification of tribbles as regulators of signal processing systems and physiological processes, including development, together with their potential involvement in diabetes and cancer, has generated considerable interest in these proteins. Tribbles have been reported to regulate the activation of some intracellular signalling pathways with roles extending from mitosis and cell activation to apoptosis and modulation of gene expression. Tribbles control the timing of mitosis in the prospective mesoderm, allowing cell-shape changes to be completed. This mechanism for coordinating cell division and cell-shape changes may have helped Drosophila to evolve its mode of rapid early development. Trib2 was identified as a downregulated transcript in leukemic cells undergoing growth arrest. Trib2-transduced bone marrow cells exhibited a growth advantage and readily established factor-dependent cell lines. Trib2-reconstituted mice uniformly developed fatal transplantable acute myelogenous leukemia (AML).

This Human TRIB2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of TRIB2 protein from the overexpression lysate was verified.
References
Seher, TC. et al., 2000, Curr Biol. 10 (11): 623-9. Keeshan, K. et al., 2006, Cancer Cell. 10 (5): 401-11. Hegedus, Z. et al., 2007, Cell Signal. 19 (2):238-50. Keeshan, K. et al., 2008, Blood Cells Mol Dis. 40 (1): 119-21. Cvetkovic, LV. et al., 2010, J Clin Invest. 120 (3): 713-9.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,801 Da
NCBI Official Full Name
tribbles homolog 2
NCBI Official Synonym Full Names
tribbles pseudokinase 2
NCBI Official Symbol
TRIB2
NCBI Official Synonym Symbols
C5FW; TRB2; GS3955
NCBI Protein Information
tribbles homolog 2
UniProt Protein Name
Tribbles homolog 2
Protein Family
UniProt Gene Name
TRIB2
UniProt Synonym Gene Names
TRB-2
UniProt Entry Name
TRIB2_HUMAN

NCBI Description

This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

TRB2: Interacts with MAPK kinases and regulates activation of MAP kinases. Does not display kinase activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; Trbl family

Chromosomal Location of Human Ortholog: 2p24.3

Cellular Component: cytoplasm; cytoskeleton; nucleus

Molecular Function: mitogen-activated protein kinase kinase binding; nucleotide binding; protein kinase activity; protein kinase inhibitor activity; transcription factor binding; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity

Biological Process: negative regulation of fat cell differentiation; negative regulation of interleukin-10 biosynthetic process; negative regulation of protein kinase activity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of MAP kinase activity

Research Articles on TRIB2

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Product Notes

The TRIB2 trib2 (Catalog #AAA8114082) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 580aa. AAA Biotech's TRIB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TRIB2 trib2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Asn34 3. It is sometimes possible for the material contained within the vial of "TRIB2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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