CD131 Cell Lysate | CSF2RB cell lysate
Human CD131 HEK293 Overexpression Lysate
Gene Names
CSF2RB; CD131; IL3RB; IL5RB; SMDP5; CDw131
Applications
Western Blot
Synonyms
CD131; Human CD131 HEK293 Overexpression Lysate; Human CD131/CSF2RB/IL3RB/IL5RB HEK293 Cell Lysate (WB positive control); colony stimulating factor 2 receptor; beta; low-affinity (granulocyte-macrophage); Human CD131 Overexpression Lysate; Human CDw131 Overexpression Lysate; Human IL3RB Overexpression Lysate; Human IL5RB Overexpression Lysate; Human SMDP5 Overexpression Lysate; CSF2RB cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
438aa
Sequence
Met1-Trp443
Applicable Applications for CSF2RB cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the extracellular domain (Met 1-Trp 443) of human CSF2RB (NP_000386.1) expressed, fused with a polyhistidine-tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CSF2RB cell lysate
Colony stimulating factor 2 receptor, beta, low-affinity (CSF2RB) also known as CD131 antigen (CD131), cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5 receptor common beta-chain, interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3 receptor, beta (IL3RB), is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this protein have been reported to be associated with protein alveolar proteinosis (PAP). CD131 belongs to the type I cytokine receptor family. The cluster of differentiation (cluster of designation) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. Defects in CD131/CSF2RB are the cause of pulmonary surfactant metabolism dysfunction type 5 (SMDP5). SMDP5 is a rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
This Human CD131 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD131 protein from the overexpression lysate was verified.
This Human CD131 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD131 protein from the overexpression lysate was verified.
References
Selleri S, et al. (2008) GM-CSF/IL-3/IL-5 receptor common beta chain (CD131) expression as a biomarker of antigen-stimulated CD8+ T cells. J Transl Med. 6:17.Woodcock, et al. (1994) Three residues in the common beta chain of the human GM-CSF, IL-3 and IL-5 receptors are essential for GM-CSF and IL-5 but not IL-3 high affinity binding and interact with Glu21 of GM-CSF. EMBO J. 13(21): 5176-85.Dirksen U, et al. (1997) Human pulmonary alveolar proteinosis associated with a defect in GM-CSF/IL-3/IL-5 receptor common beta chain expression. J Clin Invest. 100(9): 2211-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,336 Da
NCBI Official Full Name
cytokine receptor common subunit beta
NCBI Official Synonym Full Names
colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
NCBI Official Symbol
CSF2RB
NCBI Official Synonym Symbols
CD131; IL3RB; IL5RB; SMDP5; CDw131
NCBI Protein Information
cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta-chain; GM-CSF/IL-3/IL-5 receptor common beta subunit; colony-stimulating factor-2 receptor, beta, low-affinity; interleukin 3 receptor/granulocyte-macrophage colony stimulating f
UniProt Protein Name
Cytokine receptor common subunit beta
Protein Family
UniProt Gene Name
CSF2RB
UniProt Synonym Gene Names
IL3RB; IL5RB
UniProt Entry Name
IL3RB_HUMAN
NCBI Description
The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP). [provided by RefSeq, Jul 2008]
Uniprot Description
CSF2RB: a common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. A type I cytokine family of receptor. Defects are a cause of congenital pulmonary alveolar proteinosis (PAP) [MIM:265120]. PAP is an autosomal recessive fatal respiratory disease.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: granulocyte macrophage colony-stimulating factor receptor complex; integral to plasma membrane; plasma membrane
Molecular Function: protein binding; interleukin-5 receptor activity; interleukin-3 receptor activity; receptor activity
Biological Process: response to lipopolysaccharide; respiratory gaseous exchange; signal transduction
Disease: Surfactant Metabolism Dysfunction, Pulmonary, 5
Research Articles on CSF2RB
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Product Notes
The CSF2RB csf2rb (Catalog #AAA8113917) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 438aa. AAA Biotech's CD131 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CSF2RB csf2rb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Trp44 3. It is sometimes possible for the material contained within the vial of "CD131, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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