CD131 Recombinant Protein | CSF2RB recombinant protein

CD131 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8118638

• Gene Names: CSF2RB; CD131; IL3RB; IL5RB; SMDP5; CDw131
• Purity: >97 % as determined by SDS-PAGE
• Synonyms: CD131; CD131 Protein; Human; Recombinant (His Tag); Human CD131/CSF2RB/IL3RB/IL5RB Protein (His Tag); colony stimulating factor 2 receptor; beta; low-affinity (granulocyte-macrophage); CDw131 Protein; IL3RB Protein; IL5RB Protein; SMDP5 Protein; CSF2RB recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 438aa
• Sequence: Met1-Trp443

• Species: Human
• Tag: C-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Trp17
• Protein Construction: |A DNA sequence encoding the extracellular domain (Met 1-Trp 443) of human CSF2RB (NP_000386.1) expressed, fused with a polyhistidine-tag at the C-terminus
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD131 at 10ug/ml (100ul/well) can bind human Fc-GMCSF. The EC50 of human Fc-GMCSF is 250-500ng/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human CD131 at 10 ug/ml (100 ul/well) can bind human Fc-GMCSF (Cat: 10015-H01H), The EC50 of human Fc-GMCSF (Cat: 10015-H01H) is 250-500 ng/ml.)

Related Product Information for CSF2RB recombinant protein

Colony stimulating factor 2 receptor, beta, low-affinity (CSF2RB) also known as CD131 antigen (CD131), cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5 receptor common beta-chain, interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3 receptor, beta (IL3RB), is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this protein have been reported to be associated with protein alveolar proteinosis (PAP). CD131 belongs to the type I cytokine receptor family. The cluster of differentiation (cluster of designation) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. Defects in CD131/CSF2RB are the cause of pulmonary surfactant metabolism dysfunction type 5 (SMDP5). SMDP5 is a rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.

References

Selleri S, et al. (2008) GM-CSF/IL-3/IL-5 receptor common beta chain (CD131) expression as a biomarker of antigen-stimulated CD8+ T cells. J Transl Med. 6:17.Woodcock, et al. (1994) Three residues in the common beta chain of the human GM-CSF, IL-3 and IL-5 receptors are essential for GM-CSF and IL-5 but not IL-3 high affinity binding and interact with Glu21 of GM-CSF. EMBO J. 13(21): 5176-85.Dirksen U, et al. (1997) Human pulmonary alveolar proteinosis associated with a defect in GM-CSF/IL-3/IL-5 receptor common beta chain expression. J Clin Invest. 100(9): 2211-7.

NCBI and Uniprot Product Information

• NCBI GI #: 4559408
• NCBI GeneID: 1439
• NCBI Accession #: NP_000386.1
• NCBI GenBank Nucleotide #: NM_000395.2
• UniProt Accession #: P32927; Q5JZI1; Q6ICE0
• Molecular Weight: 97,336 Da
• NCBI Official Full Name: cytokine receptor common subunit beta
• NCBI Official Synonym Full Names: colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
• NCBI Official Symbol: CSF2RB
• NCBI Official Synonym Symbols: CD131; IL3RB; IL5RB; SMDP5; CDw131
• NCBI Protein Information: cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta-chain; GM-CSF/IL-3/IL-5 receptor common beta subunit; colony-stimulating factor-2 receptor, beta, low-affinity; interleukin 3 receptor/granulocyte-macrophage colony stimulating f
• UniProt Protein Name: Cytokine receptor common subunit beta
• Protein Family: Cytokine receptor
• UniProt Gene Name: CSF2RB
• UniProt Synonym Gene Names: IL3RB; IL5RB
• UniProt Entry Name: IL3RB_HUMAN

NCBI Description

The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP). [provided by RefSeq, Jul 2008]

Uniprot Description

CSF2RB: a common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. A type I cytokine family of receptor. Defects are a cause of congenital pulmonary alveolar proteinosis (PAP) [MIM:265120]. PAP is an autosomal recessive fatal respiratory disease.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: granulocyte macrophage colony-stimulating factor receptor complex; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; interleukin-5 receptor activity; interleukin-3 receptor activity; receptor activity

Biological Process: response to lipopolysaccharide; respiratory gaseous exchange; signal transduction

Disease: Surfactant Metabolism Dysfunction, Pulmonary, 5