Rabbit anti-Rat ECM1 Polyclonal Antibody | anti-ECM1 antibody

Anti-ECM1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8113047

• Reactivity: Rat
• Applications: ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: ECM1; Polyclonal Antibody; Anti-ECM1 Antibody; Rabbit Polyclonal; ECM1 Antibody; Rabbit PAb; Antigen Affinity Purified; extracellular matrix protein 1; anti-ECM1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat ECM1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ECM1 antibody: ELISA (EIA)
• Application Notes: ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Rat ECM1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rat ECM1 (rR ECM1). ECM1 specific IgG was purified by Rat ECM1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-ECM1 antibody

Extracellular matrix protein 1 (ECM1) is a secreted glycoprotein and playing a pivotal role in endochondral bone formation, angiogenesis, and tumour biology. Three splice variants have been identified: ECM1a (540 aa) is most widely expressed, with highest expression in the placenta and heart; ECM1b (415 aa) is differentiation-dependent expressed and found only in tonsil and associated with suprabasal keratinocytes; ECM1c (559 aa) accounts for approximately 15% of skin ECM1. Although ECM1 is not tumor specific, is significantly elevated in many malignant epithelial tumors and is suggested as a possible trigger for angiogenesis, tumor progression and malignancies. It also has been shown to regulate endochondral bone formation, skeletal development and tissue remodeling.

References

Oyama N, et al. (2003) Autoantibodies to extracellular matrix protein 1 in lichen sclerosus. Lancet. 362(9378): 118-23.Chan I, et al. (2004) Rapid diagnosis of lipoid proteinosis using an anti-extracellular matrix protein 1 (ECM1) antibody. J Dermatol Sci. 35(2): 151-3.Lupo I, et al. (2005) A novel mutation of the extracellular matrix protein 1 gene (ECM1) in a patient with lipoid proteinosis (Urbach-Wiethe disease) from Sicily. Br J Dermatol. 153(5): 1019-22.Sander CS, et al. (2006) Expression of extracellular matrix protein 1 (ECM1) in human skin is decreased by age and increased upon ultraviolet exposure. Br J Dermatol. 154(2): 218-24.

NCBI and Uniprot Product Information

• NCBI GI #: 55742713
• NCBI GeneID: 116662
• NCBI Accession #: NP_446334.1
• NCBI GenBank Nucleotide #: NM_053882.1
• UniProt Accession #: Q62894; Q5U2S9
• Molecular Weight: 63,249 Da
• NCBI Official Full Name: extracellular matrix protein 1
• NCBI Official Synonym Full Names: extracellular matrix protein 1
• NCBI Official Symbol: Ecm1
• NCBI Protein Information: extracellular matrix protein 1
• UniProt Protein Name: Extracellular matrix protein 1
• Protein Family: Extracellular matrix protein
• UniProt Gene Name: Ecm1
• UniProt Entry Name: ECM1_RAT

NCBI Description

mouse homolog is an extracellular protein found in a stromal osteogenic cell line [RGD, Feb 2006]

Uniprot Description

ECM1: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Defects in ECM1 are the cause of lipoid proteinosis (LiP); also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix

Molecular Function: enzyme binding; interleukin-2 receptor binding; laminin binding; protease binding; protein C-terminus binding; signal transducer activity

Biological Process: angiogenesis; biomineral formation; inflammatory response; negative regulation of bone mineralization; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of peptidase activity; ossification; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of T-helper 2 type immune response; regulation of transcription from RNA polymerase II promoter; signal transduction