ECM1 active protein

ECM1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122690

• Gene Names: Ecm1; p85; AI663821
• Purity: >88% as determined by SDS-PAGE
• Synonyms: ECM1; ECM1 Protein; Mouse; Recombinant (His Tag); Mouse ECM1 Protein (His Tag); AI663821 Protein; p85 Protein; extracellular matrix protein 1; ECM1 active protein

• Host: HEK293 Cells
• Purity/Purification: >88% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu559

• Species: Mouse
• Activity: Measured by the ability of the immobilized protein to support the adhesion of HFF Human skin fibroblast cells. When 5 × 10E4 cells/well are added to mECM1-His coated plates (2.5ug/mL and 100uL/well), approximately >45 % will adhere specifically after 90 minutes at 37 degree C.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 20
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse ECM1 isoform 1 (Q61508-1)(Met 1-Glu 559) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ECM1 active protein

Background: Extracellular matrix protein 1 (ECM1) is a secreted glycoprotein and playing a pivotal role in endochondral bone formation, angiogenesis, and tumour biology. Three splice variants have been identified: ECM1a (540 aa) is most widely expressed, with highest expression in the placenta and heart; ECM1b (415 aa) is differentiation-dependent expressed and found only in tonsil and associated with suprabasal keratinocytes; ECM1c (559 aa) accounts for approximately 15% of skin ECM1. Although ECM1 is not tumor specific, is significantly elevated in many malignant epithelial tumors and is suggested as a possible trigger for angiogenesis, tumor progression and malignancies. It also has been shown to regulate endochondral bone formation, skeletal development and tissue remodeling.

References

Oyama N, et al. (2003) Autoantibodies to extracellular matrix protein 1 in lichen sclerosus. Lancet. 362(9378): 118-23.Chan I, et al. (2004) Rapid diagnosis of lipoid proteinosis using an anti-extracellular matrix protein 1 (ECM1) antibody. J Dermatol Sci. 35(2): 151-3.Lupo I, et al. (2005) A novel mutation of the extracellular matrix protein 1 gene (ECM1) in a patient with lipoid proteinosis (Urbach-Wiethe disease) from Sicily. Br J Dermatol. 153(5): 1019-22.Sander CS, et al. (2006) Expression of extracellular matrix protein 1 (ECM1) in human skin is decreased by age and increased upon ultraviolet exposure. Br J Dermatol. 154(2): 218-24.

NCBI and Uniprot Product Information

• NCBI GI #: 170295832
• NCBI GeneID: 13601
• NCBI Accession #: NP_031925.2
• NCBI GenBank Nucleotide #: NM_007899.2
• UniProt Accession #: Q61508; Q3U5Q5
• Molecular Weight: 48,357 Da
• NCBI Official Full Name: extracellular matrix protein 1 isoform 1
• NCBI Official Synonym Full Names: extracellular matrix protein 1
• NCBI Official Symbol: Ecm1
• NCBI Official Synonym Symbols: p85; AI663821
• NCBI Protein Information: extracellular matrix protein 1
• UniProt Protein Name: Extracellular matrix protein 1
• Protein Family: Extracellular matrix protein
• UniProt Gene Name: Ecm1

Uniprot Description

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity ().