Immunoprecipitation (IP)
(rat UCHL3 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-rat UCHL3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-rat UCHL3 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa)
Rabbit anti-Rat UCHL3 Polyclonal Antibody | anti-UCHL3 antibody
Anti-UCHL3 Antibody, Rabbit Polyclonal
Gene Names
Uchl3; RGD1561196
Reactivity
Rat
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
UCHL3; Polyclonal Antibody; Anti-UCHL3 Antibody; Rabbit Polyclonal; UCHL3 Antibody; Rabbit PAb; Antigen Affinity Purified; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); anti-UCHL3 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rat UCHL3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-UCHL3 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
ELISA: 1:5000-1:10,000
IP: 0.2-1uL/mg of lysate
ELISA: 1:5000-1:10,000
IP: 0.2-1uL/mg of lysate
Immunogen
Recombinant Rat UCHL3 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Rat UCHL3 (rR UCHL3). UCHL3 specific IgG was purified by Rat UCHL3 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(rat UCHL3 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-rat UCHL3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-rat UCHL3 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa)
Immunoprecipitation (IP)
(rat UCHL3 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-rat UCHL3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-rat UCHL3 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa)
Western Blot (WB)
(Anti-UCHL3 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:23 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-UCHL3 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:23 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-UCHL3 antibody
Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase that belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.
References
Wood, M.A. et al., 2005, Hippocampus 15 (5):610-21. Kwon, J. et al., 2006, Exp Anim 55 (1):35-43. Setsuie, R. et al., 2009, Neurochem Int 54 (5-6):314-21. Setsuie, R. et al., 2010, Neurochem Int 56 (8):911-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,124 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L3
NCBI Official Synonym Full Names
ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
NCBI Official Symbol
Uchl3
NCBI Official Synonym Symbols
RGD1561196
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L3; UCH-L3; ubiquitin thioesterase L3
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L3
Protein Family
UniProt Gene Name
Uchl3
UniProt Synonym Gene Names
UCH-L3
UniProt Entry Name
UCHL3_RAT
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Product Notes
The UCHL3 uchl3 (Catalog #AAA8112746) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-UCHL3 Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UCHL3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:5000-1:10,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the UCHL3 uchl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UCHL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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