Immunohistochemistry (IHC)
(Immunochemical staining of mouse CST6 in mouse skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Mouse CST6 Polyclonal Antibody | anti-CST6 antibody
Anti-CST6 Antibody, Rabbit Polyclonal
Gene Names
Cst6; ichq; N28197; 1110017E11Rik
Reactivity
Mouse
Applications
ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
CST6; Polyclonal Antibody; Anti-CST6 Antibody; Rabbit Polyclonal; Cystatin E/CST6 Antibody; Rabbit PAb; Antigen Affinity Purified; cystatin E/M; Anti-1110017E11Rik Antibody; Anti-ichq Antibody; Anti-N28197 Antibody; anti-CST6 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse Cystatin-E/CST6/Cystatin-M/Cystatin-6
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CST6 antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IHC-P: 1:500-1:2000
Immunogen
Recombinant Mouse Cystatin-E/CST6/Cystatin-M/Cystatin-6 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Cystatin-E/CST6/Cystatin-M/Cystatin-6 (rM Cystatin-E/CST6/Cystatin-M/Cystatin-6). Cystatin-E/CST6/Cystatin-M/Cystatin-6 specific IgG was purified by Mouse Cystatin-E/CST6/Cystatin-M/Cystatin-6 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse CST6 in mouse skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse CST6 in mouse skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-CST6 antibody
Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17 kDa) and an unglycosylated (14 kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene.
References
Ritonja A., et al., (1985), Amino acid sequence of the intracellular cysteine proteinase inhibitor cystatin B from human liver. Biochem. Biophys. Res. Commun. 131:1187-1192.Pennacchio L.A., et al., (1996), Mutations in the gene encoding cystatin B in progressive myoclonus epilepsy (EPM1).Science 271:1731-1734.Ghaemmaghami S., et al., (2003), Global analysis of protein expression in yeast.Nature 425:737-741.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,796 Da
NCBI Official Full Name
cystatin-M
NCBI Official Synonym Full Names
cystatin E/M
NCBI Official Symbol
Cst6
NCBI Official Synonym Symbols
ichq; N28197; 1110017E11Rik
NCBI Protein Information
cystatin-M; cystatin M; harlequin ichthyosis; harlequin ichthysosis
UniProt Protein Name
Cystatin E/M
Protein Family
UniProt Gene Name
Cst6
UniProt Entry Name
Q9D1B1_MOUSE
Uniprot Description
CST6: Shows moderate inhibition of cathepsin B but is not active against cathepsin C. Belongs to the cystatin family.
Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide
Cellular Component: cornified envelope
Biological Process: epidermis development
Research Articles on CST6
Similar Products
Product Notes
The CST6 cst6 (Catalog #AAA8112153) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CST6 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CST6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000. Researchers should empirically determine the suitability of the CST6 cst6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CST6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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