Rabbit anti-Mouse DDC Polyclonal Antibody | anti-DDC antibody

Anti-DDC Antibody, Rabbit Polyclonal

Cat. Num. AAA8112081

• Gene Names: Ddc; Aadc
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: DDC; Polyclonal Antibody; Anti-DDC Antibody; Rabbit Polyclonal; DOPA Decarboxylase/DDC Antibody; Rabbit PAb; Antigen Affinity Purified; dopa decarboxylase (aromatic L-amino acid decarboxylase); Anti-Aadc Antibody; anti-DDC antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse DOPA Decarboxylase/DDC
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-DDC antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:1000-1:4000
• Immunogen: Recombinant Mouse DOPA Decarboxylase/DDC protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse DOPA Decarboxylase/DDC. DOPA Decarboxylase/DDC specific IgG was purified by Mouse DOPA Decarboxylase/DDC affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse DDC in mouse brain with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Western Blot (WB)

(Anti-DDC rabbit polyclonal antibody at 1:500 dilutionLane A: PC12 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDaObserved band size:53 kDa)

Related Product Information for anti-DDC antibody

Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3, 4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.

Product Categories/Family for anti-DDC antibody

Neurotransmitter Associated Enzymes

References

Ichinose, H. et al., 1989, Biochem. Biophys. Res. Commun. 164: 1024-1030. Lisa, J. S. et al., 1992, Genomics 13: 469-471. Moore, P. S. et al., 1996, Biochem. J. 315:249-256. Bertoldi, M. et al., 2003, Biochim. Biophys. Acta. 1647:42-47. Vassilacopoulou, D. et al., 2004, Neurochem. Res. 29: 1817-1823. Ma, J.Z., et al., 2005, Hum. Mol. Genet. 14: 1691-1698.

NCBI and Uniprot Product Information

• NCBI GI #: 299522784
• NCBI GeneID: 13195
• NCBI Accession #: NP_001177377.1
• NCBI GenBank Nucleotide #: NM_001190448.1
• UniProt Accession #: O88533
• Molecular Weight: 53,874 Da
• NCBI Official Full Name: aromatic-L-amino-acid decarboxylase
• NCBI Official Synonym Full Names: dopa decarboxylase
• NCBI Official Symbol: Ddc
• NCBI Official Synonym Symbols: Aadc
• NCBI Protein Information: aromatic-L-amino-acid decarboxylase
• UniProt Protein Name: Aromatic-L-amino-acid decarboxylase
• Protein Family: Aromatic-L-amino-acid decarboxylase
• UniProt Gene Name: Ddc
• UniProt Synonym Gene Names: AADC; DDC
• UniProt Entry Name: DDC_MOUSE