Rabbit anti-Mouse DLL4 Monoclonal Antibody | anti-DLL4 antibody

Recombinant Anti-DLL4 Antibody, Rabbit Monoclonal

Cat. Num. AAA8111859

• Gene Names: Dll4; Delta4
• Reactivity: Mouse
• Applications: ELISA
• Purity: Protein A
• Synonyms: DLL4; Monoclonal Antibody; Recombinant Anti-DLL4 Antibody; Rabbit Monoclonal; DLL4/Delta-like 4 Antibody; Rabbit MAb; delta-like 4 (Drosophila); Anti-Delta4 Antibody; anti-DLL4 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 004
• Specificity: Mouse DLL4/Delta-like 4
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-DLL4 antibody: ELISA (EIA) (Cap)
• Application Notes: ELISA (Cap): This antibody will detect Mouse DLL4/Delta-like 4 in ELISA pair set.; In a sandwich ELISA, it can be used as capture antibody.
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse DLL4/Delta-like 4 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse DLL4/Delta-like 4 (rM DLL4/Delta-like 4).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-DLL4 antibody

Delta-like protein 4 (DLL4, Delta4), a type I membrane-bound Notch ligand, is one of five known Notch ligands in mammals and interacts predominantly with Notch 1, which has a key role in vascular development. Recent studies yield substantial insights into the role of DLL4 in angiogenesis. DLL4 is induced by vascular endothelial growth factor (VEGF) and acts downstream of VEGF as a 'brake' on VEGF-induced vessel growth, forming an autoregulatory negative feedback loop inactivating VEGF. DLL4 is downstream of VEGF signaling and its activation triggers a negative feedback that restrains the effects of VEGF. Attenuation of DLL4/Notch signaling results in chaotic vascular network with excessive branching and sprouting. DLL4 is widely distributed in tissues other than vessels including many malignancies. Furthermore, the molecule is internalized on binding its receptor and often transported to the nucleus. In pathological conditions, such as cancer, DLL4 is up-regulated strongly in the tumour vasculature. Blockade of DLL4-mediated Notch signaling strikingly increases nonproductive angiogenesis, but significantly inhibits tumor growth in preclinical mouse models. In preclinical studies, blocking of DLL4/Notch signaling is associated with a paradoxical increase in tumor vessel density, yet causes marked growth inhibition due to functionally defective vasculature. Thus, DLL4 blockade holds promise as an additional strategy for angiogenesis-based cancer therapy.

Product Categories/Family for anti-DLL4 antibody

Cancer Drug Targets Notch Pathway

References

Yan M, et al. (2007) Delta-like 4/Notch signaling and its therapeutic implications. Clin Cancer Res. 13(24): 7243-6.Sainson RC, et al. (2007) Anti-Dll4 therapy: can we block tumour growth by increasing angiogenesis? Trends Mol Med. 13(9): 389-95.Martinez JC, et al. (2009) Nuclear and membrane expression of the angiogenesis regulator delta-like ligand 4 (DLL4) in normal and malignant human tissues. Histopathology. 54(5): 598-606.Li JL, et al. (2010) Targeting DLL4 in tumors shows preclinical activity but potentially significant toxicity. Future Oncol. 6(7): 1099-103.

NCBI and Uniprot Product Information

• NCBI GI #: 244799228
• NCBI GeneID: 54485
• NCBI Accession #: NP_062327.2
• NCBI GenBank Nucleotide #: NM_019454.3
• UniProt Accession #: Q9JI71; Q9DBU9; Q9JHZ7
• Molecular Weight: 74,991 Da
• NCBI Official Full Name: delta-like protein 4
• NCBI Official Synonym Full Names: delta-like 4 (Drosophila)
• NCBI Official Symbol: Dll4
• NCBI Official Synonym Symbols: Delta4
• NCBI Protein Information: delta-like protein 4
• UniProt Protein Name: Delta-like protein 4
• Protein Family: Delta-like protein
• UniProt Gene Name: Dll4
• UniProt Synonym Gene Names: Delta4
• UniProt Entry Name: DLL4_MOUSE

Uniprot Description

DLL4: Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4. Binds to Notch-1 and Notch-4. Expressed in vascular endothelium.

Protein type: Cell development/differentiation; Membrane protein, integral

Cellular Component: integral to plasma membrane

Molecular Function: Notch binding; protein binding

Biological Process: angiogenesis; blood vessel remodeling; negative regulation of blood vessel endothelial cell migration; negative regulation of cell proliferation; negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter; Notch signaling pathway; patterning of blood vessels; positive regulation of Notch signaling pathway; regulation of neurogenesis; T cell differentiation