Rabbit anti-Mouse Ephrin B2 Polyclonal Antibody | anti-EFNB2 antibody

Anti-Ephrin B2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8111826

• Gene Names: Efnb2; Epl5; ELF-2; Eplg5; Htk-L; Lerk5; LERK-5; NLERK-1
• Reactivity: Mouse
• Applications: ELISA
• Purity: Protein A
• Synonyms: Ephrin B2; Polyclonal Antibody; Anti-Ephrin B2 Antibody; Rabbit Polyclonal; Ephrin-B2/EFNB2 Antibody; Rabbit PAb; ephrin-B2; Anti-ELF-2 Antibody; Anti-Epl5 Antibody; Anti-Eplg5 Antibody; Anti-Htk-L Antibody; Anti-LERK-5 Antibody; Anti-Lerk5 Antibody; Anti-NLERK-1 Antibody; anti-EFNB2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse EFNB2/Ephrin-B2
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-EFNB2 antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Mouse EFNB2 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse EFNB2 (rM EFNB2). Total IgG was purified by Protein A affinity chromatography .
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-EFNB2 antibody

EphrinB2 also known as EFNB2 is a member of the ephrin family. EphrinB2 is involved in establishing arterial versus venous identity and perhaps in anastamosing arterial and venous vessels at their junctions. The transmembrane-associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB2 expression progressively extends from the arterial endothelium to surrounding smooth muscle cells and to pericytes, suggesting that ephrin-B2 may play an important role during formation of the arterial muscle wall.

References

Wang HU, et al. (1998) Molecular distinction and angiogenic interaction between embryonic arteries and veins revealed by ephrin-B2 and its receptor Eph-B4. Cell. 93(5): 741-53.Gale NW, et al. (2001) Ephrin-B2 selectively marks arterial vessels and neovascularization sites in the adult, with expression in both endothelial and smooth-muscle cells. Dev Biol. 230(2): 151-60.Shin D, et al. (2001) Expression of ephrinB2 identifies a stable genetic difference between arterial and venous vascular smooth muscle as well as endothelial cells, and marks subsets of microvessels at sites of adult neovascularization. Dev Biol. 230(2): 139-50.

NCBI and Uniprot Product Information

• NCBI GI #: 31542597
• NCBI GeneID: 13642
• NCBI Accession #: NP_034241.2
• NCBI GenBank Nucleotide #: NM_010111.5
• UniProt Accession #: P52800
• Molecular Weight: 37,202 Da
• NCBI Official Full Name: ephrin-B2
• NCBI Official Synonym Full Names: ephrin B2
• NCBI Official Symbol: Efnb2
• NCBI Official Synonym Symbols: Epl5; ELF-2; Eplg5; Htk-L; Lerk5; LERK-5; NLERK-1
• NCBI Protein Information: ephrin-B2
• UniProt Protein Name: Ephrin-B2
• Protein Family: Ephrin
• UniProt Gene Name: Efnb2
• UniProt Synonym Gene Names: Elf2; Epl5; Eplg5; Htkl; Lerk5; LERK-5; HTK-L
• UniProt Entry Name: EFNB2_MOUSE

Uniprot Description

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.