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Western Blot (WB)
(Anti-PRDX5 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse lung tissue lysateLane B: Mouse brain tissue lysateLane C: Mouse liver tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDa)
Rabbit anti-Mouse Peroxiredoxin 5 Polyclonal Antibody | anti-PRDX5 antibody
Anti-Peroxiredoxin 5 Antibody, Rabbit Polyclonal
Gene Names
Prdx5; AOPP; PrxV; Pmp20; Prdx6; AOEB166
Reactivity
Mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
Peroxiredoxin 5; Polyclonal Antibody; Anti-Peroxiredoxin 5 Antibody; Rabbit Polyclonal; Peroxiredoxin 5/PRDX5 Antibody; Rabbit PAb; Antigen Affinity Purified; peroxiredoxin 5; Anti-AOEB166 Antibody; Anti-AOPP Antibody; Anti-Pmp20 Antibody; Anti-Prdx6 Antibody; Anti-PrxV Antibody; anti-PRDX5 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse Peroxiredoxin 5/PRDX5
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PRDX5 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC-P: 1:200-1:1000
IP: 0.5-2uL/mg of lysate
ELISA: 1:5000-1:10,000
IHC-P: 1:200-1:1000
IP: 0.5-2uL/mg of lysate
Immunogen
Recombinant Mouse Peroxiredoxin 5/PRDX5 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Peroxiredoxin 5/PRDX5 (rM Peroxiredoxin 5/PRDX5). Peroxiredoxin 5/PRDX5 specific IgG was purified by Mouse Peroxiredoxin 5/PRDX5 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PRDX5 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse lung tissue lysateLane B: Mouse brain tissue lysateLane C: Mouse liver tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDa)
Western Blot (WB)
(Anti-PRDX5 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse lung tissue lysateLane B: Mouse brain tissue lysateLane C: Mouse liver tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse PRDX5 in mouse kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse PRDX5 in mouse kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse PRDX5 in mouse colon with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse PRDX5 in mouse colon with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(Mprdx5 was immunoprecipitated using: Lane A:0.5 mg Mouse lung tissue Lysate 4 uL anti-Mprdx5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Mprdx5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 22 kDa Observed band size :16 kDa)
Immunoprecipitation (IP)
(Mprdx5 was immunoprecipitated using: Lane A:0.5 mg Mouse lung tissue Lysate 4 uL anti-Mprdx5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Mprdx5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 22 kDa Observed band size :16 kDa)
Related Product Information for anti-PRDX5 antibody
Peroxiredoxin-5, also known as Alu corepressor 1, Antioxidant enzyme B166, Liver tissue 2D-page spot 71B, Peroxisomal antioxidant enzyme, Thioredoxin peroxidase PMP20, Thioredoxin reductase, PRDX5 and ACR1, is cytoplasm protein that belongs to the peroxiredoxin 2 family. Peroxiredoxin-5/PRDX5 reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Peroxiredoxin-5/PRDX5 is involved in intracellular redox signaling. The Peroxiredoxins/Prx are a family of 25 kDa peroxidases that can reduce H2O2 using an electron from thioredoxin (Trx) or other substances. The mammalian Peroxiredoxins/Prx family is divided into six groups (PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, PRDX6) on the basis of homology of amino acid sequences. They are located in the cytosol and play a role in the cell signaling system. All six mammalian peroxiredoxins are expressed in the lung. Peroxiredoxins/Prx is overexpressed in breast cancer tissues to a great extent suggesting that Peroxiredoxins/Prx has a proliferative effect and may be related to cancer development or progression.
References
Seo M.S., et al., 2000, J. Biol. Chem. 275: 20346-54. Declercq J.-P., et al., 2001, J. Mol. Biol. 311:751-9. Noh, D.Y. et al., 2001, Anticancer Res. 21 (3B): 2085-90. Schremmer, B. et al., 2007, Subcell Biochem. 44 :317-44.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,897 Da
NCBI Official Full Name
peroxiredoxin-5, mitochondrial
NCBI Official Synonym Full Names
peroxiredoxin 5
NCBI Official Symbol
Prdx5
NCBI Official Synonym Symbols
AOPP; PrxV; Pmp20; Prdx6; AOEB166
NCBI Protein Information
peroxiredoxin-5, mitochondrial; PLP; prx-V; peroxiredoxin 6; peroxiredoxin V; thioredoxin reductase; antioxidant enzyme B166; thioredoxin peroxidase PMP20; peroxisomal antioxidant enzyme; peroxisomal membrane protein 20; liver tissue 2D-page spot 2D-0014I
UniProt Protein Name
Peroxiredoxin-5, mitochondrial
Protein Family
UniProt Gene Name
Prdx5
UniProt Synonym Gene Names
Prdx6; AOEB166; Prx-V
UniProt Entry Name
PRDX5_MOUSE
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Product Notes
The PRDX5 prdx5 (Catalog #AAA8111736) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Peroxiredoxin 5 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Peroxiredoxin 5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC-P: 1:200-1:1000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the PRDX5 prdx5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peroxiredoxin 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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