Rabbit anti-Mouse Tissue Factor Monoclonal Antibody | anti-F3 antibody

Recombinant Anti-Tissue Factor Antibody, Rabbit Monoclonal

Cat. Num. AAA8111533

• Gene Names: F3; TF; Cf3; Cf-3; CD142; AA409063
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Protein A
• Synonyms: Tissue Factor; Monoclonal Antibody; Recombinant Anti-Tissue Factor Antibody; Rabbit Monoclonal; Coagulation Factor III/Tissue Factor/CD142 Antibody; Rabbit MAb; coagulation factor III (thromboplastin; tissue factor); Anti-AA409063 Antibody; Anti-CD142 Antibody; Anti-Cf-3 Antibody; Anti-Cf3 Antibody; Anti-TF Antibody; anti-F3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 034
• Specificity: Mouse Coagulation Factor III/Tissue Factor/CD142/F3
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-F3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse Coagulation Factor III/Tissue Factor/CD142/F3 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Coagulation Factor III/Tissue Factor/CD142/F3 (rM Coagulation Factor III/Tissue Factor/CD142/F3).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-F3 rabbit monoclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDaObserved band size:56 kDa)

Related Product Information for anti-F3 antibody

Tissue factor (TF), also known as coagulation factor III, F3, and CD142, is a single-pass type I membrane protein which belongs to the tissue factor family. Tissue factor is one of the proteins that participate in hemostatic and inflammatory processes. Activated monocytes present in the liver increase expression of tissue factor, and while accumulating in the organ they can intensify inflammation. Tissue factor is the protein that activates the blood clotting system by binding to, and activating, the plasma serine protease, factor VIIa, following vascular injury. Tissue factor is not only the main physiological initiator of normal blood coagulation, but is also important in the natural history of solid malignancies in that it potentiates metastasis and angiogenesis and mediates outside-in signalling. Tissue factor is expressed constitutively by many tissues which are not in contact with blood and by other cells upon injury or activation; the latter include endothelial cells, tissue macrophages, and peripheral blood monocytes. Coagulation Factor III is a transmembrane glycoprotein that localizes the coagulation serine protease factor VII/VIIa (FVII/VIIa) to the cell surface. The primary function of TF is to activate the clotting cascade. The TF:FVIIa complex also activates cells by cleavage of a G-protein coupled receptor called protease-activated receptor 2 (PAR2). TF is expressed by tumor cells and contributes to a variety of pathologic processes, such as thrombosis, metastasis, tumor growth, and tumor angiogenesis. As a key regulator of haemostasis and angiogenesis, it is also involved in the pathology of several diseases, including cardiovascular, inflammatory and neoplastic conditions.

Product Categories/Family for anti-F3 antibody

Serine Proteases and Regulators

References

Morrissey JH. (2004) Tissue factor: a key molecule in hemostatic and nonhemostatic systems. Int J Hematol. 79(2): 103-8.Milsom C, et al. (2008) Tissue factor and cancer. Pathophysiol Haemost Thromb. 36(3-4): 160-76.Kasthuri RS, et al. (2009) Role of tissue factor in cancer. J Clin Oncol. 27(29): 4834-8.

NCBI and Uniprot Product Information

• NCBI GI #: 170172540
• NCBI GeneID: 14066
• NCBI Accession #: NP_034301.3
• NCBI GenBank Nucleotide #: NM_010171.3
• UniProt Accession #: P20352
• Molecular Weight: 32,935 Da
• NCBI Official Full Name: tissue factor
• NCBI Official Synonym Full Names: coagulation factor III
• NCBI Official Symbol: F3
• NCBI Official Synonym Symbols: TF; Cf3; Cf-3; CD142; AA409063
• NCBI Protein Information: tissue factor
• UniProt Protein Name: Tissue factor
• Protein Family: Tissue factor
• UniProt Gene Name: F3
• UniProt Synonym Gene Names: Cf-3; Cf3; TF

NCBI Description

This gene encodes a membrane-bound glycoprotein that forms the primary physiological initiator of the blood coagulation process following vascular damage. The encoded protein binds to coagulation factor VIIa and the ensuing complex catalyzes the proteolytic activation of coagulation factors IX and X. Mice lacking encoded protein die in utero resulting from massive hemorrhaging in both extraembryonic and embryonic vessels. A severe deficiency of the encoded protein in mice results in impaired uterine homeostasis, shorter life spans due to spontaneous fatal hemorrhages and cardiac fibrosis. [provided by RefSeq, Aug 2015]

Uniprot Description

F3: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF-alpha, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response. Lung, placenta and pancreas. Belongs to the tissue factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3 G1|3 52.94 cM

Cellular Component: cell surface; cytoplasm; extracellular matrix; extracellular space; integral to membrane; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; phospholipid binding; protease binding; serine-type endopeptidase activity

Biological Process: activation of plasma proteins during acute inflammatory response; blood coagulation; caspase activation; cytokine and chemokine mediated signaling pathway; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell migration; protein processing