Rabbit anti-Human Tissue Factor Polyclonal Antibody | anti-F3 antibody

Anti-Tissue Factor Antibody, Rabbit Polyclonal

Cat. Num. AAA8106754

• Gene Names: F3; TF; TFA; CD142
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Tissue Factor; Polyclonal Antibody; Anti-Tissue Factor Antibody; Rabbit Polyclonal; Coagulation Factor III/Tissue Factor/CD142 Antibody; Rabbit PAb; Antigen Affinity Purified; coagulation factor III (thromboplastin; tissue factor); Anti-CD142 Antibody; Anti-coagulation factor 3 Antibody; Anti-coagulation factor III Antibody; Anti-TF Antibody; Anti-TFA Antibody; anti-F3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Coagulation Factor III/Tissue Factor/CD142
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-F3 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human Coagulation Factor III/Tissue Factor/CD142 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Coagulation Factor III/Tissue Factor/CD142 (rh Coagulation Factor III/Tissue Factor/CD142). Coagulation Factor III/Tissue Factor/CD142 specific IgG was purified by Human Coagulation Factor III/Tissue Factor/CD142 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(F3 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate2 uL anti-F3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-F3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 50 kDaObserved band size: 50 kDa)

Western Blot (WB)

(Anti-F3 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDaObserved band size:48 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-F3 antibody

Tissue factor (TF), also known as coagulation factor III, F3, and CD142, is a single-pass type I membrane protein which belongs to the tissue factor family. Tissue factor is one of the proteins that participate in hemostatic and inflammatory processes. Activated monocytes present in the liver increase expression of tissue factor, and while accumulating in the organ they can intensify inflammation. Tissue factor is the protein that activates the blood clotting system by binding to, and activating, the plasma serine protease, factor VIIa, following vascular injury. Tissue factor is not only the main physiological initiator of normal blood coagulation, but is also important in the natural history of solid malignancies in that it potentiates metastasis and angiogenesis and mediates outside-in signalling. Tissue factor is expressed constitutively by many tissues which are not in contact with blood and by other cells upon injury or activation; the latter include endothelial cells, tissue macrophages, and peripheral blood monocytes. Coagulation Factor III is a transmembrane glycoprotein that localizes the coagulation serine protease factor VII/VIIa (FVII/VIIa) to the cell surface. The primary function of TF is to activate the clotting cascade. The TF:FVIIa complex also activates cells by cleavage of a G-protein coupled receptor called protease-activated receptor 2 (PAR2). TF is expressed by tumor cells and contributes to a variety of pathologic processes, such as thrombosis, metastasis, tumor growth, and tumor angiogenesis. As a key regulator of haemostasis and angiogenesis, it is also involved in the pathology of several diseases, including cardiovascular, inflammatory and neoplastic conditions.

Product Categories/Family for anti-F3 antibody

Serine Proteases and Regulators

References

Morrissey JH. (2004) Tissue factor: a key molecule in hemostatic and nonhemostatic systems. Int J Hematol. 79(2): 103-8.Milsom C, et al. (2008) Tissue factor and cancer. Pathophysiol Haemost Thromb. 36(3-4): 160-76.Kasthuri RS, et al. (2009) Role of tissue factor in cancer. J Clin Oncol. 27(29): 4834-8.

NCBI and Uniprot Product Information

• NCBI GI #: 296010912
• NCBI GeneID: 2152
• NCBI Accession #: NP_001171567.1
• NCBI GenBank Nucleotide #: NM_001178096.1
• UniProt Accession #: P13726; Q6FHG2; Q86WH4; D3DT47
• Molecular Weight: 33,068 Da
• NCBI Official Full Name: tissue factor isoform 2
• NCBI Official Synonym Full Names: coagulation factor III (thromboplastin, tissue factor)
• NCBI Official Symbol: F3
• NCBI Official Synonym Symbols: TF; TFA; CD142
• NCBI Protein Information: tissue factor
• UniProt Protein Name: Tissue factor
• Protein Family: Tissue factor
• UniProt Gene Name: F3
• UniProt Synonym Gene Names: TF
• UniProt Entry Name: TF_HUMAN

NCBI Description

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

F3: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF-alpha, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response. Lung, placenta and pancreas. Belongs to the tissue factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p22-p21

Cellular Component: extracellular matrix; extracellular space; cell surface; integral to membrane; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protease binding; phospholipid binding

Biological Process: caspase activation; activation of blood coagulation via clotting cascade; positive regulation of protein kinase B signaling cascade; blood coagulation, extrinsic pathway; positive regulation of angiogenesis; activation of plasma proteins during acute inflammatory response; positive regulation of positive chemotaxis; cytokine and chemokine mediated signaling pathway; positive regulation of endothelial cell proliferation; blood coagulation; positive regulation of cell migration