Rabbit anti-Mouse VE-Cadherin Monoclonal Antibody | anti-CDH5 antibody

Recombinant Anti-VE-Cadherin Antibody, Rabbit Monoclonal

Cat. Num. AAA8111175

• Gene Names: Cdh5; 7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Protein A
• Synonyms: VE-Cadherin; Monoclonal Antibody; Recombinant Anti-VE-Cadherin Antibody; Rabbit Monoclonal; VE-Cadherin/CD144/CDH5 Antibody; Rabbit MAb; cadherin 5; type 2 (vascular endothelium); Anti-7B4 Antibody; Anti-AA408225 Antibody; Anti-Cd144 Antibody; Anti-VE-Cad Antibody; Anti-Vec Antibody; Anti-VEcad Antibody; Anti-VECD Antibody; anti-CDH5 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 311
• Specificity: Mouse VE-Cadherin/CD144/CDH5
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CDH5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse VE-Cadherin/CD144/CDH5 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse VE-Cadherin/CD144/CDH5 (rM VE-Cadherin/CD144/CDH5).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CDH5 rabbit monoclonal antibody at 1:500Lane A: Rat lung tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:88 kDaObserved band size:88 kDa)

Related Product Information for anti-CDH5 antibody

Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, an endothelial specific cell-cell adhesion molecule, plays a pivotal role in the formation, maturation and remodeling of the vascular wall. VE-Cadherin is widely considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. This specificity of VE-cadherin for vascular endothelial cells is important not only in blood and lymph vessel biology and medicine, but also for cell-type-based identifies, notably those of metastatic tumors. As a classical cadherin, VE-Cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Mechanisms that regulate VE-cadherin-mediated adhesion are important for the control of vascular permeability and leukocyte extravasation. In addition to its adhesive functions, VE-Cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Consequently, VE-cadherin is essential during embryonic angiogenesis.

Product Categories/Family for anti-CDH5 antibody

Hemangioblast Markers Cardiac Stem Cell Markers; Canonical Wnt Pathway

References

Taveau JC, et al. (2008) Structure of artificial and natural VE-cadherin-based adherens junctions. Biochem Soc Trans. 36(Pt 2): 189-93.Vestweber D. (2008) VE-cadherin: the major endothelial adhesion molecule controlling cellular junctions and blood vessel formation. Arterioscler Thromb Vasc Biol. 28(2): 223-32.Gavard J. (2009) Breaking the VE-cadherin bonds. FEBS Lett. 583(1): 1-6.Vestweber D, et al. (2009) Cell adhesion dynamics at endothelial junctions: VE-cadherin as a major player. Trends Cell Biol. 19(1): 8-15.Boda-Heggemann J, et al. (2009) Beyond vessels: occurrence and regional clustering of vascular endothelial (VE-)cadherin-containing junctions in non-endothelial cells. Cell Tissue Res. 335(1): 49-65.

NCBI and Uniprot Product Information

• NCBI GI #: 38490561
• NCBI GeneID: 12562
• NCBI Accession #: NP_033998.2
• NCBI GenBank Nucleotide #: NM_009868.4
• UniProt Accession #: P55284; O35542
• Molecular Weight: 87,903 Da
• NCBI Official Full Name: cadherin-5 preproprotein
• NCBI Official Synonym Full Names: cadherin 5
• NCBI Official Symbol: Cdh5
• NCBI Official Synonym Symbols: 7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225
• NCBI Protein Information: cadherin-5
• UniProt Protein Name: Cadherin-5
• Protein Family: Cadherin
• UniProt Gene Name: Cdh5
• UniProt Synonym Gene Names: VE-cadherin
• UniProt Entry Name: CADH5_MOUSE

Uniprot Description

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: adherens junction; cell junction; cell surface; external side of plasma membrane; intercellular junction; tight junction

Molecular Function: beta-catenin binding; protein binding; protein phosphatase binding; receptor binding

Biological Process: blood vessel maturation; cell-cell adhesion; intercellular junction assembly; negative regulation of cell proliferation; transforming growth factor beta receptor signaling pathway