Western Blot (WB)
(Anti-TYSND1 rabbit polyclonal antibody at 1:500 dilution Lane A: U251MG Whole Cell Lysate Lane B: NCI-H460 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:59 kDa Observed band size:53 kDa)
Rabbit anti-Human TYSND1 Polyclonal Antibody | anti-TYSND1 antibody
Anti-TYSND1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
TYSND1; Polyclonal Antibody; Anti-TYSND1 Antibody; Rabbit Polyclonal; TYSND1 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-NET41 Antibody; anti-TYSND1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TYSND1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TYSND1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
E Coli-derived Human TYSND1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human TYSND1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-TYSND1 rabbit polyclonal antibody at 1:500 dilution Lane A: U251MG Whole Cell Lysate Lane B: NCI-H460 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:59 kDa Observed band size:53 kDa)
Western Blot (WB)
(Anti-TYSND1 rabbit polyclonal antibody at 1:500 dilution Lane A: U251MG Whole Cell Lysate Lane B: NCI-H460 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:59 kDa Observed band size:53 kDa)
Related Product Information for anti-TYSND1 antibody
Tysnd1 (trypsin domain containing 1) is localized in peroxisomes and cleaves PTS2-containing prethiolase Acaa1, PTS1-containing proteins Acox1 (acyl-CoA oxidase 1, palmitoyl), Hsd17b4 (hydroxysteroid (17-beta) dehydrogenase 4) and ScpX, the longer mRNA product of gene Scp2 (sterol carrier protein 2, liver). Tysnd1 is responsible for both removal of PTS2-containing leader peptide from prethiolase and for specific processing of all PTS1 proteins involved in the peroxisomal beta-oxidation pathway of fatty acids. Tysnd1-mediated site-specific proteolysis is a part of regulatory mechanisms that control the peroxisomal beta-oxidation of fatty acids. Tysnd1 is necessary to mediate the physiological functions of PTS2-containing substrates.
References
Okumoto K, et al. (2011) Two proteases, trypsin domain-containing 1 (tysnd1) and peroxisomal lon protease (pslon), cooperatively regulate fatty acid beta-oxidation in peroxisomal matrix. J Biol Chem 286 (52): 44367-44379.Mizuno Y, et al. (2013) Tysnd1 deficiency in mice interferes with the peroxisomal localization of pts2 enzymes, causing lipid metabolic abnormalities and male infertility. PLoS Genet 9 (2): e1003286.Kurochkin IV, et al. (2007) Novel peroxisomal protease tysnd1 processes pts1-and pts2-containing enzymes involved in beta-oxidation of fatty acids. Embo j 26 (3): 835-845.
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Product Notes
The TYSND1 (Catalog #AAA8109627) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TYSND1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TYSND1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the TYSND1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TYSND1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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