Immunohistochemistry (IHC)
(Immunochemical staining of human PHTF2 in human gallbladder with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human PHTF2 Polyclonal Antibody | anti-PHTF2 antibody
Anti-PHTF2 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
PHTF2; Polyclonal Antibody; Anti-PHTF2 Antibody; Rabbit Polyclonal; PHTF2 Antibody; Rabbit PAb; Antigen Affinity Purified; putative homeodomain transcription factor 2; anti-PHTF2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PHTF2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PHTF2 antibody
Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
IHC-P: 1:100-1:300
ICC/IF: 1:100-1:500
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human PHTF2 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PHTF2 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human PHTF2 in human gallbladder with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PHTF2 in human gallbladder with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PHTF2 in human liver with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PHTF2 in human liver with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of PHTF2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PHTF2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus and cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of PHTF2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PHTF2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus and cytoplasm.)
Related Product Information for anti-PHTF2 antibody
PHTF2 (Putative Homeodomain Transcription Factor 2) is a Protein Coding gene. The PHTF2 gene, located on 7q11.23-q21.11, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, fruit fly, and frog. PHTF2 is widely expressed in the lymph node, appendix, and other tissues, and may play a role in transcription regulation. PHTF2 was identified as a hub gene in the protein-protein interaction (PPI) network and could regulate lipids metabolism significantly affected the tumorigenesis of GC cells. Diseases associated with PHTF2 include Dysgerminoma Of the Ovary. An important paralog of this gene is PHTF1. 231 organisms have orthologs with the human gene PHTF2.
References
Shaffer JR, et al. (2013) Gwas of dental caries patterns in the permanent dentition. J Dent Res 92 (1): 38-44.Manuel A, et al. (2000) Molecular characterization of a novel gene family (phtf) conserved from drosophila to mammals. Genomics 64 (2): 216-220.Chi Y, et al. (2020) Phtf2 regulates lipids metabolism in gastric cancer. Aging (Albany NY) 12 (8): 6600-6610.
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Product Notes
The PHTF2 (Catalog #AAA8108900) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PHTF2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHTF2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). IHC-P: 1:100-1:300 ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the PHTF2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHTF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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