Western Blot (WB)
(Anti-FAM3C rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:25 kDa Observed band size:25 kDa)
Rabbit anti-Human FAM3C Polyclonal Antibody | anti-FAM3C antibody
Anti-FAM3C Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IHC-P: 1:2500-1:10,000
IP: 4-8uL/mg of lysate
Western Blot (WB)
(Anti-FAM3C rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:25 kDa Observed band size:25 kDa)
Western Blot (WB)
(Anti-FAM3C rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:25 kDa Observed band size:25 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human FAM3C in human brain with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human FAM3C in human brain with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunoprecipitation (IP)
(FAM3C was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-FAM3C rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-FAM3C rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 25 kDa Observed band size: 25 kDa)
Immunoprecipitation (IP)
(FAM3C was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-FAM3C rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-FAM3C rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 25 kDa Observed band size: 25 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the family with sequence similarity 3 (FAM3) family and encodes a secreted protein with a GG domain. A change in expression of this protein has been noted in pancreatic cancer-derived cells. [provided by RefSeq, Mar 2010]
Uniprot Description
FAM3C: May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition. Belongs to the FAM3 family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 7q31
Cellular Component: Golgi apparatus; cytoplasmic membrane-bound vesicle; extracellular region
Molecular Function: cytokine activity
Biological Process: multicellular organismal development
Research Articles on FAM3C
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Product Notes
The FAM3C fam3c (Catalog #AAA8107657) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FAM3C Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAM3C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC-P: 1:2500-1:10,000 IP: 4-8uL/mg of lysate. Researchers should empirically determine the suitability of the FAM3C fam3c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAM3C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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