Rabbit anti-Human DCUN1D2 Monoclonal Antibody | anti-DCUN1D2 antibody

Recombinant Anti-DCUN1D2 Antibody, Rabbit Monoclonal

Cat. Num. AAA8107555

• Gene Names: DCUN1D2; C13orf17
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A
• Synonyms: DCUN1D2; Monoclonal Antibody; Recombinant Anti-DCUN1D2 Antibody; Rabbit Monoclonal; DCUN1D2/DCN1-like protein 2 Antibody; Rabbit MAb; DCN1; defective in cullin neddylation 1; domain containing 2; Anti-C13orf17 Antibody; anti-DCUN1D2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 001
• Specificity: Human DCUN1D2/DCN1-like protein 2
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-DCUN1D2 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human DCUN1D2/DCN1-like protein 2 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human DCUN1D2/DCN1-like protein 2 (rh DCUN1D2/DCN1-like protein 2).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-DCUN1D2 rabbit monoclonal antibody at 1:500 dilutionLane A: HCT116 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:30 kDa)

Immunoprecipitation (IP)

(DCUN1D2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg HuT78 Whole Cell Lysate2 uL anti-DCUN1D2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DCUN1D2 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 30 kDa)

NCBI and Uniprot Product Information

• NCBI GI #: 62122952
• NCBI GeneID: 55208
• NCBI Accession #: NP_001014305.1
• NCBI GenBank Nucleotide #: NM_001014283.1
• UniProt Accession #: Q6PH85; Q5JSA5; Q5JSA6; Q5JSA7; Q9NVJ1; Q9NXR6
• Molecular Weight: 21,467 Da
• NCBI Official Full Name: DCN1-like protein 2
• NCBI Official Synonym Full Names: DCN1, defective in cullin neddylation 1, domain containing 2
• NCBI Official Symbol: DCUN1D2
• NCBI Official Synonym Symbols: C13orf17
• NCBI Protein Information: DCN1-like protein 2; DCUN1 domain-containing protein 2; defective in cullin neddylation protein 1-like protein 2
• UniProt Protein Name: DCN1-like protein 2
• Protein Family: DCN1-like protein
• UniProt Gene Name: DCUN1D2
• UniProt Synonym Gene Names: C13orf17; DCUN1L2
• UniProt Entry Name: DCNL2_HUMAN

Uniprot Description

DCUN1D2: May contribute to neddylation of cullin components of SCF-type E3 ubiquitin ligase complexes. Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 13q34